Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)
Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)
Thermo Scientific™

Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)

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Catalog NumberNo. of Reactions
K0241200 reactions
K02434,000 Reactions
K02421,000 Reactions
Catalog number K0241
Price (USD)
287.00
Each
Add to cart
No. of Reactions:
200 reactions
Price (USD)
287.00
Each
Add to cart
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Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers (see Resources for a compatibility chart).

Highlights

SpecificityMaxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity—detects low copy number targets
Wide linear range—accurate quantification across 9 orders of magnitude
Reproducibility and convenience—ready-to-use 2x master mix

Applications

• Gene expression
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Maxima SYBR Green/Fluorescein qPCR Master Mix (2x) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer supplemented with fluorescein passive reference dye. Supplied with nuclease-free water.

Related Products
Maxima SYBR Green/ROX qPCR Master Mix (2X)
Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)
Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)BioRad iCycler iQ, BioRad MyiQ, BioRad iQ5
FormatTube
No. of Reactions200 reactions
PolymeraseTaq DNA Polymerase
Product LineMaxima
Product TypeReal Time PCR SYBR Master Mix
Quantity200 reactions
Sample TypeDNA, dsDNA
Sufficient For200 Reactions of 25 μL
Volume2 x 1.25 mL
Concentration2X
Detection MethodSYBR
For Use With (Application)Gene Expression
GC-Rich PCR PerformanceHigh
PCR MethodqPCR
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
Contains:
• 2 x 1.25 mL Maxima SYBR Green/Fluorescein qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer with fluorescein passive reference dye; sufficient for 200 x 25 μL reactions.
• 2 x 1.25 mL Nuclease-Free Water

Store at -20°C.