Invitrogen™

iBright™ Prestained Protein Ladder

Catalog number: LC5615
Invitrogen™

iBright™ Prestained Protein Ladder

Catalog number: LC5615
Catalog Number
LC5615
Unit Size
2 x 250 µL
Price (USD)
Price: 289.00
Your Price:
Quantity
Catalog NumberUnit SizePrice (USD)Quantity
LC56152 x 250 µL
Price: 289.00
Your Price:
Product Overview
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iBright Prestained Protein Ladder contains twelve recombinant proteins, ten (11 to 250 kDa) that are blue-stained and fluor-labeled for direct and near-IR fluorescent visualization and protein sizing, and two (30 kDa and 80 kDa) that are unstained and contain IgG binding sites to bind the primary and secondary antibodies used for chemiluminescent and fluorescent detection of the target protein. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

Compare and view all other protein standards and ladders ›

Product features
• Fluorescent visualization—detect the 10 stained proteins using the 670 nm red laser or 700 nm channel of a fluorescence imager
• Western blot confirmation—detect the two unstained proteins (30 kDa and 80 kDa) using the detection system for the target protein

Applications
• Western blotting: detection of the two unstained bands via the detection method used for the target protein. Compatible with chemiluminescent substrates and fluorescent secondary antibodies.
• Protein sizing on SDS-polyacrylamide gels, visualization of proteins during electrophoresis and transfer, and visualization of proteins during fluorescence imaging (ten blue-stained bands)
• Protein size estimation: protein sizing on SDS-polyacrylamide gels and on western blot using near-infrared (NIR) fluorescence imagers

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Colorimetric, NIR Fluorescence, and User defined detection system (2 bands)
Number of Markers
12
Product Type Specs
Protein Ladder
Ready to Load
Yes
Size Range
11 to 250 kDa
Stain Type
1 color: Blue
Gel Compatibility
Novex™ Midi Gels, Novex™ Mini Gels, Novex™Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Gels, NuPAGE™ MES Gels, NuPAGE™ MOPS Gels, NuPAGE™ Tris-Acetate Gels, Precise™ Tris-Glycine Gels, SDS-PAGE Gels, Precise™ Tris-HEPES Gels
Molecular Weight
250, 130, 95, 80, 70, 55, 43, 34, 30, 26, 15, 11 kDa
Product Line
iBright™
Quantity
2 x 250 μL
Shipping Condition
Dry Ice
System Type
Western Blotting, SDS-PAGE

Contents & Storage

Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% (w/v) SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol

Upon receipt, store at –20°C. Product is shipped on ice.