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ATP Synthase beta Monoclonal Antibody (4.3E8.D10), Alexa Fluor™ 555
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FIGURE: 1 / 1
ATP Synthase beta Antibody (MA1-930-A555) in ICC/IF
Immunofluorescent analysis of ATP Synthase Beta (orange) in HeLa cells. The cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 3% Blocker BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a ATP Synthase beta mouse monoclonal antibody, AlexaFluor 555 conjugate (Product # MA1-930-A555), at a concentration of 5 µg/mL in blocking buffer... View More
Product Details
MA1-930-A555
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
4.3E8.D10
Immunogen
Intact rat mitochondria
Conjugate
Excitation/Emission Max
553/568 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1930A555 detects the beta subunit of ATP synthase from mouse rat and human samples. This antibody is useful as a mitochondrial marker.
Target Information
ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATP synthase F(1) complex catalytic subunit beta, mitochondrial; ATP synthase F(1) complex subunit beta, mitochondrial; ATP synthase F1 subunit beta; ATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting mitochondrial F1 complex, alpha subunit; ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit; ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide; Atp5b; epididymis secretory protein Li 271; MGC5231; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthase, H+ transporting F1 complex beta subunit; mitochondrial ATP synthetase, beta subunit; unnamed protein product
Gene Aliases: ATP5B; ATP5F1B; ATPMB; ATPSB; HEL-S-271; HUMOP2
UniProt ID: (Human) P06576, (Rat) P10719, (Mouse) P56480
Entrez Gene ID: (Human) 506, (Rat) 171374, (Mouse) 11947
nucleotide binding
protein binding
ATP binding
MHC class I protein binding
angiostatin binding
metal ion binding
proton-transporting ATP synthase activity, rotational mechanism
proton-transporting ATPase activity, rotational mechanism
calcium ion binding
ATPase activity
lipoprotein particle receptor activity
hydrolase activity
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
ATP synthase
angiogenesis
osteoblast differentiation
generation of precursor metabolites and energy
lipid metabolic process
ATP biosynthetic process
ion transport
negative regulation of cell adhesion involved in substrate-bound cell migration
ATP synthesis coupled proton transport
mitochondrial ATP synthesis coupled proton transport
positive regulation of blood vessel endothelial cell migration
ATP metabolic process
regulation of intracellular pH
cellular response to interleukin-7
hydrogen ion transmembrane transport
receptor-mediated endocytosis
ATP hydrolysis coupled proton transport
proton transport
transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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