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Invitrogen
TNFRSF14 Recombinant Rabbit Monoclonal Antibody (028)
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FIGURE: 1 / 1
TNFRSF14 Antibody (MA5-30950) in ICC/IF
Immunofluorescence staining of HVEM in Raji cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with TNFRSF14 Recombinant Rabbit Monoclonal Antibody (28) (Product # MA5-30950, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and Cell membrane.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-30950
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
028
Immunogen
Recombinant Human HVEM/TNFRSF14/CD270 Protein (Met1-Val202)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human HVEM/TNFRSF14/CD270.
Target Information
TNFRSF14 is a member of the TNF-receptor superfamily. TNFRSF14 was identified as a cellular mediator of herpes simplex virus (HSV) entry. Binding of HSV viral envelope glycoprotein D (gD) to TNFRSF14 has been shown to be part of the viral entry mechanism. The cytoplasmic region of TNFRSF14 was found to bind to several TRAF family members, which may mediate the signal transduction pathways that activate the immune response. Activation of the signal transduction pathway involving TNFRSF14 in T cells stimulates T cell proliferation and cytokine production, leading to inflammation and enhanced CTL-mediated tumor immunity, suggesting that these proteins may be useful as potential targets for controlling cellular immune responses. Multiple alternatively spliced transcript variants have been described, but the full-length nature of some of these TNFRSF14 variants have not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD40-like protein; herpes virus entry mediator A; HGNC:11912; RP3-395M20.6; sCD2710; TNFRSF14; tumo; tumor necrosis factor receptor superfamily, member 14; tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator); tumor necrosis factor receptor-like gene2; unnamed protein product
Gene Aliases: ATAR; CD270; HVEA; HVEM; LIGHTR; TR2
UniProt ID: (Human) Q92956
Entrez Gene ID: (Human) 8764
positive regulation of cytokine production involved in immune response
negative regulation of T cell cytokine production
immune response-regulating cell surface receptor signaling pathway
regulation of T cell tolerance induction to tumor cell
immune response
cell surface receptor signaling pathway
regulation of B cell proliferation
T cell costimulation
regulation of interferon-gamma production
tumor necrosis factor-mediated signaling pathway
T cell proliferation
negative regulation of T cell proliferation
negative regulation of alpha-beta T cell proliferation
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
negative regulation of T cell activation
regulation of ERK1 and ERK2 cascade
negative regulation of adaptive immune memory response
positive regulation of T cell migration
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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