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          • Primary Antibodies ›
          • c-Myc Antibodies

          Invitrogen

          Phospho-c-Myc (Thr58, Ser62) Recombinant Rabbit Monoclonal Antibody (10F1V1)

          View all (122) c-Myc antibodies

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          Cite Phospho-c-Myc (Thr58, Ser62) Recombinant Rabbit Monoclonal Antibody (10F1V1)

          Additional Information:
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          • Antibody Testing Data (1)
          Phospho-c-Myc (Thr58, Ser62) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Phospho-c-Myc (Thr58, Ser62) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

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          Phospho-c-Myc (Thr58, Ser62) Antibody (MA5-35847) in WB

          Western blot analysis of Phospho-c-Myc (Thr58, Ser62) in extracts of C6 cells. Samples were incubated with Phospho-c-Myc (Thr58, Ser62) Monoclonal antibody (Product # MA5-35847) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. C6 cells were treated by CIP (20 µL/400 µL) at 37°C for 1 hour. Lysates/proteins: 25 µg per lane. Blocking buffer:... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-c-Myc (Thr58, Ser62) Antibody in Western Blot (WB)
          Phospho-c-Myc (Thr58, Ser62) Recombinant Rabbit Monoclonal Antibody (10F1V1)

          Product Details

          MA5-35847

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Rat

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          10F1V1

          Immunogen

          A synthetic phosphorylated peptide around T58 & S62 of human c-Myc (P01106)
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol, 0.05% BSA

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2849746

          Product Specific Information

          Immunogen sequence: TPPLS

          Target Information

          The c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies have shown that c-Myc is essential for vasculogenesis and angiogenesis in tumor development, which distributes blood throughout the cells. The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation, and the deregulated expression of c-Myc induces apoptosis in different cell types. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins. c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (delta light chain); or 22 (light chain) is described in Burkitts lymphoma and other B-cell lymphoproliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: Avian myelocytomatosis viral (v-myc) oncogene homolog; avian myelocytomatosis viral oncogene homolog; bHLHe39; c Myc; c-myc; c-myc epitope tag; c-myc protein, first expressed; Class E basic helix-loop-helix protein 39; cmyc; Myc protein; Myc proto-oncogene protein; myc-related translation/localization regulatory factor; myelocytomatosis viral oncogene homolog; ORF 114; ORF 114; putative; ORF 118; Proto-oncogene c-Myc; Transcription factor p64; unnamed protein product; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog

          View more View less

          Gene Aliases: BHLHE39; c-Myc; mMyc; MRTL; MYC; MYCC; RNCMYC

          View more View less

          UniProt ID: (Human) P01106, (Rat) P09416

          View more View less

          Entrez Gene ID: (Human) 4609, (Rat) 24577

          View more View less

          Function(s)
          RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding transcription cofactor binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding ubiquitin protein ligase binding identical protein binding macromolecular complex binding protein dimerization activity E-box binding DNA-binding transcription factor binding SCF ubiquitin ligase complex binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding double-stranded DNA binding transcription factor binding protein complex binding sequence-specific DNA binding protein heterodimerization activity repressing transcription factor binding basic helix-loop-helix transcription factor
          Process(es)
          G1/S transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter MAPK cascade branching involved in ureteric bud morphogenesis B cell apoptotic process NK T cell proliferation positive regulation of mesenchymal cell proliferation positive regulation of B cell apoptotic process chromatin remodeling regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter cellular iron ion homeostasis cellular response to DNA damage stimulus cell proliferation positive regulation of cell proliferation intrinsic apoptotic signaling pathway in response to DNA damage response to radiation response to xenobiotic stimulus regulation of gene expression regulation of cell cycle process positive regulation of gene expression myotube differentiation Wnt signaling pathway rRNA metabolic process protein processing regulation of telomere maintenance positive regulation of telomere maintenance negative regulation of stress-activated MAPK cascade protein-DNA complex disassembly cellular response to UV cellular response to interferon-alpha skeletal muscle cell differentiation middle ear morphogenesis regulation of apoptotic process negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process response to alkaloid pigmentation hypermethylation of CpG island fibroblast apoptotic process negative regulation of monocyte differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation negative regulation of fibroblast proliferation skeletal system morphogenesis positive regulation of epithelial cell proliferation detection of mechanical stimulus involved in sensory perception of sound chromosome organization negative regulation of cell division positive regulation of transcription initiation from RNA polymerase II promoter negative regulation of transcription initiation from RNA polymerase II promoter ERK1 and ERK2 cascade response to growth factor cellular response to hypoxia cellular response to xenobiotic stimulus positive regulation of metanephric cap mesenchymal cell proliferation positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator positive regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of somatic stem cell population maintenance positive regulation of acinar cell proliferation acinar cell proliferation positive regulation of apoptotic signaling pathway in utero embryonic development regulation of oxidative phosphorylation glucose metabolic process transcription, DNA-templated DNA-templated transcription, initiation pyruvate transport amino acid transport activation of cysteine-type endopeptidase activity involved in apoptotic process inner mitochondrial membrane organization cell cycle arrest regulation of mitotic cell cycle glycoprotein metabolic process pathogenesis response to wounding flavonoid metabolic process response to gamma radiation negative regulation of gene expression positive regulation of mitochondrial membrane potential transformation of host cell by virus hypothalamus development negative regulation of protein binding response to estradiol cellular response to drug response to drug positive regulation of catalytic activity positive regulation of cysteine-type endopeptidase activity involved in apoptotic process canonical Wnt signaling pathway involved in positive regulation of wound healing canonical Wnt signaling pathway involved in negative regulation of apoptotic process canonical Wnt signaling pathway involved in positive regulation of apoptotic process G0 to G1 transition positive regulation of cell cycle positive regulation of glycolytic process negative regulation of glucose import lactic acid secretion canonical Wnt signaling pathway negative regulation of cell death cellular response to carbohydrate stimulus cellular response to interleukin-1 cellular response to estrogen stimulus cellular response to organic cyclic compound cellular response to hydrostatic pressure fungal-type cell wall polysaccharide metabolic process positive regulation of cellular respiration positive regulation of DNA biosynthetic process positive regulation of response to DNA damage stimulus positive regulation of ATP biosynthetic process
          It has to be done as per old AB suggested Products section.

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