Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. Also, use NuPAGE gels for optimal results during day-to-day use.
Features of NuPAGE Bis-Tris gels:
• Better protein integrity—optimized sample preparation process preserves your proteins
• Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
• Faster run times—get separation of your proteins in as little as 35 minutes
• Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperatureLearn more about all of our NuPAGE Bis-Tris gels >View migration charts ›Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.
NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer
and NuPAGE Sample Reducing Agent
. Use NuPAGE Antioxidant
in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer
to better resolve small proteins or NuPAGE MOPS SDS Running Buffer
to resolve medium- to large-size proteins.
We also provide NuPAGE Tris-Acetate gels
for separating larger proteins. For classic Laemmli-based Tris-glycine electrophoresis, we provide Novex Tris-Glycine gels
For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer
. Rapid semi-dry transfer can be done using the Pierce Power Blotter
or rapid dry transfer using the iBlot 2 Gel Transfer Device
. Alternatively, traditional wet transfer can be performed using the XCell II Blot Module
or the Mini Blot Module
. Related links Overview of 1D Protein ElectrophoresisComparison of NuPAGE Tris-Bis vs. traditional Tris-glycine gels
For Research Use Only. Not for use in diagnostic procedures.