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Invitrogen
ALKBH8 Polyclonal Antibody
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Product Details
PA5-58517
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human ALKBH8. Recombinant protein control fragment (Product #RP-97368).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Amount
10 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: LPPGLMVVEE IISSEEEKML LESVDWTEDT DNQNSQKSLK HRRVKHFGYE FHYENNNVDK DKPLSGGLPD ICESFLEKWL RKGYIKHKPD QMTINQYEPG QG
Highest antigen sequence identity to the following orthologs: Mouse - 82%, Rat - 84%.
Target Information
Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain (PubMed:20123966, PubMed:20308323). Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA (PubMed:20123966, PubMed:20308323). Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys)(PubMed:20308323). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly) (PubMed:21285950). Required for normal survival after DNA damage (PubMed:20308323). May inhibit apoptosis and promote cell survival and angiogenesis (PubMed:19293182).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AlkB homologue 8; alkB, alkylation repair homolog 8; Alkylated DNA repair protein alkB homolog 8; Alpha-ketoglutarate-dependent dioxygenase ALKBH8; probable alpha-ketoglutarate-dependent dioxygenase ABH8; S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8; S-adenosyl-L-methionine-dependent tRNA methyltransferase ALKBH8; tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8; tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ALKBH8; tRNA methyltransferase 9 homolog; tRNA methyltransferase 9 related; unnamed protein product
Gene Aliases: ABH8; ALKBH8; MRT71; TRM9; TRMT9; TRMT9A
UniProt ID: (Human) Q96BT7
Entrez Gene ID: (Human) 91801
tRNA binding
nucleic acid binding
RNA binding
catalytic activity
iron ion binding
protein binding
methyltransferase activity
zinc ion binding
S-adenosylmethionine-dependent methyltransferase activity
tRNA (uracil) methyltransferase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
transferase activity
metal ion binding
transition metal ion binding
tRNA (carboxymethyluridine(34)-5-O)-methyltransferase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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