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Invitrogen
ZNRF1 Polyclonal Antibody
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FIGURE: 1 / 4
ZNRF1 Antibody (PA5-103824) in ICC/IF
Immunofluorescent analysis of ZNRF1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and ZNRF1 polyclonal antibody (Product # PA5-103824) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-103824
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ZNRF1(Accession Q8ND25), corresponding to amino acid residues P129-K179.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total ZNRF1.
Target Information
E3 ubiquitin-protein ligase that mediates the ubiquitination of AKT1 and GLUL, thereby playing a role in neuron cells differentiation. Plays a role in the establishment and maintenance of neuronal transmission and plasticity. Regulates Schwann cells differentiation by mediating ubiquitination of GLUL. Promotes neurodegeneration by mediating 'Lys-48'-linked polyubiquitination and subsequent degradation of AKT1 in axons: degradation of AKT1 prevents AKT1-mediated phosphorylation of GSK3B, leading to GSK3B activation and phosphorylation of DPYSL2/CRMP2 followed by destabilization of microtubule assembly in axons (Probable).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC101991; nerve injury gene 283; nerve injury-induced gene 283 protein; peripheral nerve injury protein nin283; ring finger protein 42; RING-type E3 ubiquitin transferase ZNRF1; unnamed protein product; zinc and ring finger 1, E3 ubiquitin protein ligase; zinc and ring finger protein 1; zinc ring finger protein 1; zinc/RING finger protein 1
Gene Aliases: B830022L21Rik; NIN283; Rnf42; Zrfp1
UniProt ID: (Human) Q8ND25, (Mouse) Q91V17
Entrez Gene ID: (Human) 84937, (Rat) 690769, (Mouse) 170737
protein ubiquitination
positive regulation of toll-like receptor 4 signaling pathway
proteasome-mediated ubiquitin-dependent protein catabolic process
protein K48-linked ubiquitination
positive regulation of autophagosome-lysosome fusion
cytoskeleton organization
toll-like receptor 3 signaling pathway
regulation of toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
regulation of toll-like receptor 4 signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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