Western blot analysis of Phospho-RyR2 (Ser2814) in A431 cell lysate (Lane 1: treated with phospho-blocking peptide; Lane 2: treated with non-phospho-blocking peptide). Samples were incubated with Phospho-RyR2 (Ser2814) polyclonal antibody (Product # PA5-104558).

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Immunogen image

Immunogen Image Phospho-RyR2 (Ser2814) Polyclonal Antibody

Product Details

PA5-104558

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

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Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Product Specifications

Species Reactivity

Dog, Human

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human RYR2(Accession Q92736), corresponding to amino acid residues around phosphorylated Ser2814.

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

100 µg

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2816033

Product Specific Information

Antibody detects endogenous levels of RYR2 only when phosphorylated at Ser2814.

Target Information

Dihydropyridine receptor (DHPR) is a surface membrane protein critical for the excitation-contraction coupling of striated muscle. DHPR and the sarcoplasmic reticulum ryanodine receptor (RyR) are two key components of the intracellular junctions, where depolarization of the surface membrane is converted into the release of Ca2+ from internal stores. The alpha1-subunit of the DHPR contains a cytoplasmic loop which is thought to be involved in the interactions with RyR. Phosphorylation of the DHPR alpha1-subunit is also thought to play a role in the functional interaction of DHPR and RyR. Mutation in DHPR alpha1 results in excitation-contraction uncoupling, leading to muscular dysgenesis, a complete inactivity in developing skeletal muscles. Cells that do not express RyR also lack excitation-contraction coupling and exhibit a severalfold reduction in Ca2+ current density.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Cardiac muscle ryanodine receptor; Cardiac muscle ryanodine receptor-calcium release channel; cardiac ryanodine receptor 2; cardiac-type ryanodine receptor; hRYR-2; islet-type ryanodine receptor; kidney-type ryanodine receptor; Ryanodine receptor; Ryanodine receptor 2; ryanodine receptor 2 (cardiac); RYR-2; Type 2 ryanodine receptor; unnamed protein product

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Gene Aliases: ARVC2; ARVD2; RyR; RYR-2; RYR2; VACRDS; VTSIP

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UniProt ID: (Human) Q92736

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Entrez Gene ID: (Human) 6262, (Dog) 403615

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Function(s)

ion channel activity ryanodine-sensitive calcium-release channel activity calcium channel activity calcium ion binding protein binding calmodulin binding calcium-release channel activity enzyme binding protein kinase binding protein kinase A catalytic subunit binding protein kinase A regulatory subunit binding identical protein binding suramin binding ion channel binding calcium-induced calcium release activity ligand-gated ion channel

Process(es)

response to hypoxia regulation of heart rate embryonic heart tube morphogenesis left ventricular cardiac muscle tissue morphogenesis cardiac muscle hypertrophy detection of calcium ion ion transport calcium ion transport cellular calcium ion homeostasis striated muscle contraction positive regulation of heart rate regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion regulation of cardiac muscle contraction by calcium ion signaling release of sequestered calcium ion into cytosol by sarcoplasmic reticulum response to muscle activity calcium-mediated signaling response to caffeine ion transmembrane transport response to muscle stretch release of sequestered calcium ion into cytosol positive regulation of sequestering of calcium ion regulation of cytosolic calcium ion concentration establishment of localization in cell response to redox state transmembrane transport regulation of cardiac muscle contraction cardiac muscle contraction calcium ion transport into cytosol sarcoplasmic reticulum calcium ion transport calcium ion transmembrane transport cellular response to caffeine cellular response to epinephrine stimulus establishment of protein localization to endoplasmic reticulum ventricular cardiac muscle cell action potential Purkinje myocyte to ventricular cardiac muscle cell signaling cell communication by electrical coupling involved in cardiac conduction type B pancreatic cell apoptotic process calcium ion transmembrane import into cytosol positive regulation of the force of heart contraction regulation of AV node cell action potential regulation of SA node cell action potential regulation of atrial cardiac muscle cell action potential regulation of ventricular cardiac muscle cell action potential

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Phospho-RyR2 (Ser2814) Polyclonal Antibody

Phospho-RyR2 (Ser2814) Antibody (PA5-104558) in WB

Product Details

PA5-104558

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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