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Invitrogen
Mimecan Polyclonal Antibody
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FIGURE: 1 / 4
Mimecan Antibody (PA5-112635) in ICC/IF
Immunocytochemichal analysis of Mimecan in HepG2 cells using a Polyclonal antibody (Product # PA5-112635) at a dilution of 1:100. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.
Product Details
PA5-112635
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human Mimecan protein (21-298AA)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Mimecan induces ectopic bone formation in conjunction with transforming growth factor beta. Mimecan is a small proteoglycan which contains tandem leucine-rich repeats. Mimecan is encoded by the OGN gene, a member of the small leucine-rich proteoglycan (SLRP) family of proteins. Mimecan induces ectopic bone formation in conjunction with transforming growth factor beta (TGF-beta-1 or TGF-beta-2) and may regulate osteoblast differentiation. High expression of mimecan protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants of mimecan. Alternatively spliced transcript variants that encode mimecan have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: corneal keratan sulfate proteoglycan; MCN; small leucine-rich proteoglycan; Mimecan; mimecan proteoglycan; OGN; OIF; Osteoglycin; Osteoinductive factor; unnamed protein product
Gene Aliases: OG; OGN; OIF; SLRR3A
UniProt ID: (Human) P20774
Entrez Gene ID: (Human) 4969, (Rat) 291015
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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