Search
Invitrogen
Caspase 3 (Cleaved Asp175) Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 27
Caspase 3 (Cleaved Asp175) Antibody (PA5-114687) in ICC/IF
Immunocytochemistry analysis of Caspase 3 (Cleaved Asp175) in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114687) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-114687
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CASP3(Accession P42574), corresponding to amino acid residues C163-M182.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of fragment of activated Caspase 3 resulting from cleavage adjacent to Asp175.
Target Information
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: apopain; CASP-3; caspase 3, apoptosis related cysteine protease; Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; CPP-32; CPP32beta; cysteine protease CPP32; cysteine protease p32-beta; IRP; PARP cleavage protease; procaspase3; protein Yama; SCA-1; SREBP cleavage activity 1; unnamed protein product; Yama
Gene Aliases: A830040C14Rik; AC-3; CASP-3; Caspase-3; CC3; CPP-32; CPP32; CPP32-beta; CPP32B; Lice; mldy; SCA-1; Yama
UniProt ID: (Human) P42574, (Mouse) P70677, (Rat) P55213
Entrez Gene ID: (Human) 836, (Mouse) 12367, (Rat) 25402
protease binding
endopeptidase activity
aspartic-type endopeptidase activity
cysteine-type endopeptidase activity
cyclin-dependent protein serine/threonine kinase inhibitor activity
death receptor binding
protein binding
enzyme activator activity
peptidase activity
cysteine-type peptidase activity
phospholipase A2 activator activity
macromolecular complex binding
luteolysis
response to hypoxia
negative regulation of cytokine production
proteolysis
apoptotic process
axonal fasciculation
learning or memory
intrinsic apoptotic signaling pathway in response to osmotic stress
response to xenobiotic stimulus
response to glucose
response to metal ion
response to X-ray
regulation of macroautophagy
protein processing
hippocampus development
protein catabolic process
neuron differentiation
keratinocyte differentiation
erythrocyte differentiation
platelet formation
regulation of protein stability
response to cobalt ion
response to estradiol
response to lipopolysaccharide
regulation of protein localization
glial cell apoptotic process
response to tumor necrosis factor
response to nicotine
intracellular signal transduction
swimming behavior
response to hydrogen peroxide
positive regulation of apoptotic process
response to amino acid
regulation of neuron apoptotic process
positive regulation of neuron apoptotic process
response to ethanol
neurotrophin TRK receptor signaling pathway
striated muscle cell differentiation
response to glucocorticoid
protein maturation
pyroptosis
leukocyte apoptotic process
response to anesthetic
cellular response to staurosporine
apoptotic signaling pathway
intrinsic apoptotic signaling pathway
execution phase of apoptosis
regulation of synaptic vesicle cycle
synapse pruning
positive regulation of pyroptotic inflammatory response
positive regulation of beta-amyloid formation
positive regulation of apoptotic DNA fragmentation
response to insulin-like growth factor stimulus
B cell homeostasis
cellular response to DNA damage stimulus
heart development
sensory perception of sound
response to UV
response to wounding
negative regulation of B cell proliferation
interleukin-18-mediated signaling pathway
T cell homeostasis
negative regulation of apoptotic process
cell fate commitment
negative regulation of cyclin-dependent protein serine/threonine kinase activity
negative regulation of cell cycle
negative regulation of activated T cell proliferation
neuron apoptotic process
anterior neural tube closure
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
protein poly-ADP-ribosylation
response to ionizing radiation
response to auditory stimulus
animal organ development
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support