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Invitrogen

HMGB1 Polyclonal Antibody

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HMGB1 Polyclonal Antibody

Product Details

PA5-119191

Applications
Tested Dilution
Publications

Western Blot (WB)

0.01-2 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

5-20 µg/mL
-

Immunocytochemistry (ICC/IF)

5-20 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein High Mobility Group Protein 1. The antigen corresponds to amino acid range 1-215 of the target protein.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography, Protein A

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2903691

Target Information

High-mobility group box 1 protein, previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. It is now known that HMGB1 can also act extracellularly, both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction. Moreover, HMGB1 is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Amphoterin; Amphoterin antibody; DKFZp686A04236; high mobility group protein 1; high-mobility group (nonhistone chromosomal) protein 1; HMG3 antibody; hmgb 1; hmgb-1; HMGB1; RP11-550P23.1; Sulfoglucuronyl carbohydrate binding protein; unnamed protein product

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Gene Aliases: HMG-1; HMG1; HMG3; p30; SBP-1

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UniProt ID: (Human) P09429, (Mouse) P63158

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Entrez Gene ID: (Human) 3146, (Mouse) 15289

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Function(s)
four-way junction DNA binding bubble DNA binding transcription regulatory region sequence-specific DNA binding lipopolysaccharide binding phosphatidylserine binding DNA binding damaged DNA binding double-stranded DNA binding single-stranded DNA binding transcription coactivator activity transcription corepressor activity RNA binding double-stranded RNA binding single-stranded RNA binding cytokine activity integrin binding protein binding DNA binding, bending calcium-dependent protein kinase regulator activity lyase activity C-X-C chemokine binding protein kinase activator activity chemoattractant activity receptor agonist activity RAGE receptor binding RNA polymerase II sequence-specific DNA binding transcription factor binding DNA polymerase binding supercoiled DNA binding DNA-binding transcription factor binding endodeoxyribonuclease activator activity open form four-way junction DNA binding crossed form four-way junction DNA binding nucleic acid binding bent DNA binding 5S rRNA binding heparin binding peptide binding protein heterodimerization activity glycolipid binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter myeloid dendritic cell activation endothelial cell proliferation positive regulation of endothelial cell proliferation activation of innate immune response plasmacytoid dendritic cell activation macrophage activation involved in immune response dendritic cell chemotaxis inflammatory response to antigenic stimulus regulation of tolerance induction regulation of T cell mediated immune response to tumor cell DNA metabolic process DNA topological change base-excision repair double-strand break repair double-strand break repair via nonhomologous end joining DNA recombination chromatin organization chromatin remodeling inflammatory response immune response signal transduction positive regulation of cytosolic calcium ion concentration positive regulation of autophagy negative regulation of endothelial cell migration negative regulation of RNA polymerase II transcriptional preinitiation complex assembly neuron projection development heterochromatin assembly DNA geometric change positive regulation of mismatch repair negative regulation of interferon-gamma production positive regulation of chemokine production positive regulation of interferon-alpha production positive regulation of interferon-beta production positive regulation of interleukin-1 beta production positive regulation of interleukin-1 production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production V(D)J recombination positive regulation of toll-like receptor 2 signaling pathway positive regulation of toll-like receptor 4 signaling pathway positive regulation of toll-like receptor 9 signaling pathway T-helper 1 cell activation endothelial cell chemotaxis positive regulation of activated T cell proliferation positive regulation of apoptotic process apoptotic cell clearance negative regulation of CD4-positive, alpha-beta T cell differentiation positive regulation of DNA binding positive regulation of MAPK cascade positive regulation of blood vessel endothelial cell migration negative regulation of blood vessel endothelial cell migration T-helper 1 cell differentiation positive regulation of innate immune response positive regulation of cell differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade positive regulation of viral entry into host cell regulation of viral process positive chemotaxis regulation of DNA metabolic process positive regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide positive regulation of monocyte chemotactic protein-1 production positive regulation of monocyte chemotaxis positive regulation of wound healing neutrophil clearance positive regulation of NIK/NF-kappaB signaling positive regulation of sprouting angiogenesis regulation of hemopoiesis positive regulation of vascular endothelial cell proliferation positive regulation of chemokine (C-X-C motif) ligand 2 production negative regulation of apoptotic cell clearance regulation of nucleotide-excision repair positive regulation of dendritic cell differentiation eye development positive regulation of protein phosphorylation positive regulation of mesenchymal cell proliferation DNA repair chemotaxis cell proliferation lung development positive regulation of cell migration response to lipopolysaccharide positive regulation of myeloid cell differentiation positive regulation of glycogen catabolic process positive regulation of mitotic cell cycle induction of positive chemotaxis response to glucocorticoid cellular response to cytokine stimulus cellular response to interleukin-7
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