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Invitrogen
HSP90 alpha Polyclonal Antibody
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FIGURE: 1 / 10
HSP90 alpha Antibody (PA5-16341) in WB
CRISPR-Cas9 mediated genome editing ofHSP90 alpha (as confirmed by next generation sequencing) was achieved by using LentiArray™ Lentiviral sgRNA (Product # A32042, AssayID CRISPR610567_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Fig (a) Western blot analysis of HSP90 alpha was performed by loading 30 µg of HeLa Wild Type (Lane 1), HeLa Cas9 (Lane 2) and HeLa Cas9 cells transduced with HSP90 alpha Lentiviral sgRNA (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Prod... View More
Product Details
PA5-16341
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic 11-mer peptide, corresponding to aa 2-12 (PEETQTQDQPM-Cys) from the N-terminus end of mouse heat shock protein 90alpha (hsp90alpha)
Conjugate
Form
Liquid
Concentration
2 mg/mL
Amount
1 mg
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA5-16341 targets Heat Shock Protein 90 alpha in immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation, and western blot applications and shows reactivity with Human samples.
The PA5-16341 immunogen is a synthetic 11-mer peptide, corresponding to aa 2-12 (PEETQTQDQPM-Cys) from the N-terminus end of mouse heat shock protein 90alpha (hsp90alpha).
This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
Target Information
Heat shock proteins (HSP) are proteins that are expressed in response to various biological stress, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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