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Invitrogen
LC3A (Cleaved Gly120) Polyclonal Antibody
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FIGURE: 1 / 7
LC3A (Cleaved Gly120) Antibody (PA5-35196) in ICC/IF
Immunocytochemistry analysis of LC3A (Cleaved Gly120) in HeLa cells. Samples were incubated with LC3A (Cleaved Gly120) polyclonal antibody (Product # PA5-35196) using a dilution of 1:25 followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG at a dilution of 1:400 (green). Cells were 4% paraformaldehyde-fixed and 0.1% Triton X-100 permeabilized. Immunofluorescence image showing vesicles staining on Hela... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-35196
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 89-120 amino acids from human Cleaved LC3A or LC3B
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
200 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with bovine and rat based on sequence homology.
Target Information
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: autophagy-related protein LC3 A; autophagy-related ubiquitin-like modifier LC3 A; LC3-I; LC3-like protein 1; LC3I; MAP1 light chain 3-like protein 1; MAP1A/1B light chain 3 A; MAP1A/MAP1B LC3 A; MAP1A/MAP1B light chain 3 A; Microtubule-associated protein 1 light chain; microtubule-associated proteins 1A/1B light chain 3; microtubule-associated proteins 1A/1B light chain 3A; unnamed protein product
Gene Aliases: 1010001H21Rik; 4922501H04Rik; ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3
UniProt ID: (Human) Q9H492, (Mouse) Q91VR7
Entrez Gene ID: (Human) 84557, (Mouse) 66734
autophagosome assembly
mitophagy
mitophagy
autophagy
cellular response to nitrogen starvation
JNK cascade
cellular response to starvation
response to iron(II) ion
response to lead ion
macroautophagy
response to nutrient levels
cellular response to amino acid starvation
p38MAPK cascade
SMAD protein signal transduction
cellular response to hydrogen peroxide
cellular response to copper ion
cellular response to oxygen-glucose deprivation
autophagosome maturation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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