Search

  • Contact Us
  • Quick Order

Invitrogen

HIF-2 alpha Polyclonal Antibody

Advanced Verification
View all (33) HIF-2 alpha antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Additional Information:

{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}

Product Details

PA5-85845

Applications
Tested Dilution
Publications

Western Blot (WB)

1:100-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-
Product Specifications

Species Reactivity

Fish, Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A peptide derived from the mouse/human HIF2 alpha.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2792965

Product Specific Information

Keep as concentrated solution.

Positive Control: Hypoxic lysates, HepG2 (1% O2 treatment for 24hr and using Urea buffer), Myc-DDK human HIF2A-transfected 293T.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

HIF-2 alpha (Epas1) is a transcription factor involved in the induction of genes regulated by oxygen. HIF-2 alpha contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. It also regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of the lungs. Mutations in this gene are associated with erythrocytosis familial type 4.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: basic-helix-loop-helix-PAS protein MOP2; class E basic helix-loop-helix protein 73; EPAS-1; Hif like protein; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF-2-alpha; HIF-related factor; HIF1 alpha-like factor; HIF1alpha-like factor; HIF2-alpha; HLF (HIF1alpha-like factor); HRF; hypoxia inducible factor 2, alpha subunit; hypoxia inducible factor 2a; hypoxia inducible transcription factor 2alpha; hypoxia-inducible factor 2 alpha; hypoxia-inducible factor 2-alpha; member of PAS protein 2; mHLF; PAS domain-containing protein 2; unnamed protein product

View more View less

Gene Aliases: bHLHe73; ECYT4; HIF-2 alpha; HIF-2alpha; HIF2 alpha; HIF2A; HLF; HRF; MOP2; PASD2

View more View less

UniProt ID: (Human) Q99814, (Rat) Q9JHS1, (Mouse) P97481

View more View less

Entrez Gene ID: (Human) 2034, (Rat) 29452, (Mouse) 13819

View more View less

Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcription coactivator binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding sequence-specific DNA binding protein heterodimerization activity protein dimerization activity RNA polymerase II sequence-specific DNA binding transcription factor binding cobalt ion binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter angiogenesis response to hypoxia embryonic placenta development blood vessel remodeling regulation of heart rate regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter response to oxidative stress mitochondrion organization heart development visual perception gene expression hemopoiesis cell differentiation erythrocyte differentiation lung development oxygen homeostasis norepinephrine metabolic process norepinephrine biosynthetic process surfactant homeostasis positive regulation of transcription from RNA polymerase II promoter cell maturation myoblast fate commitment positive regulation of multicellular organismal process multicellular organismal iron ion homeostasis cellular response to lipopolysaccharide cellular response to interleukin-1 cellular response to hypoxia circulatory system development positive regulation of cold-induced thermogenesis positive regulation of dopamine biosynthetic process positive regulation of lung alveolus development regulation of protein neddylation signal transduction tube development positive regulation of transcription, DNA-templated tissue remodeling
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support