Search
Invitrogen
AMBRA1 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-88053
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.22 mg/mL
Amount
122 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: U-87MG, HeLa, Jurkat, MCF7; Cellular Location: Cytoplasmic vesicle, autophagosome
Immunogen Sequence: GAAHTSSPQP STSRGLLPEA GQLAERGLSP RTASWDQPGT PGREPTQPTL PSSSPVPIPV SLPSAEGPTL HCELTNNNHL LDGGSSRGDA AGPRGEPRNR
Target Information
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death. Furthermore, down-regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: activating molecule in Beclin1-regulated autophagy; autophagy/beclin 1 regulator 1; ischemia related factor NYW-1; unnamed protein product
Gene Aliases: 2310079H06Rik; A130023A14; D030051N19Rik; mKIAA1736; Nyw1
UniProt ID: (Mouse) A2AH22
Entrez Gene ID: (Mouse) 228361, (Rat) 59319
autophagosome assembly
G1/S transition of mitotic cell cycle
protein polyubiquitination
mitophagy
regulation of transcription from RNA polymerase II promoter
autophagy
negative regulation of cell proliferation
cellular response to starvation
positive regulation of autophagy
negative regulation of cardiac muscle cell apoptotic process
protein ubiquitination
neural tube development
response to nutrient levels
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of neuron apoptotic process
positive regulation of regulatory T cell differentiation
positive regulation of protein kinase B signaling
response to mitochondrial depolarisation
positive regulation of mitophagy
positive regulation of free ubiquitin chain polymerization
regulation of G1/S transition of mitotic cell cycle
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support