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Invitrogen
Human ATP4A (aa 878-915) Control Fragment Recombinant Protein
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Product Details
RP-95785
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
TDYFTAMAQEGWFPLLCVGLRAQWEDHHLQDLQDSYGQ
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (95%), Rat (95%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-58673. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
The ATP4A gene encodes the alpha subunit of the gastric H+/K+ ATPase, an enzyme crucial for gastric acid secretion in the stomach. This proton pump resides in the parietal cells of the gastric mucosa and is responsible for exchanging potassium ions (K+) for hydrogen ions (H+), effectively acidifying the stomach contents. ATP4A plays a significant role in maintaining the acidic environment necessary for digestion and defense against pathogens. The ATPase's activity is tightly regulated by various factors, including hormones like gastrin and histamine, to ensure optimal stomach function. Mutations in ATP4A can lead to disorders related to gastric acidity, such as hypochlorhydria (low stomach acid), which can impact digestion and absorption of nutrients. Understanding ATP4A's function and its regulatory mechanisms is essential for developing treatments for conditions like peptic ulcer disease and gastroesophageal reflux disease, where gastric acid secretion is disrupted.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATPase H+/K+ transporting alpha subunit; ATPase, H+/K+ exchanging, alpha polypeptide; ATPase, H+/K+ transporting, alpha polypeptide; epididymis secretory sperm binding protein; gastric H+/K+ ATPase alpha subunit; gastric H,K-ATPase catalytic subunit; gastric hydrogen-potassium ATPase; proton pump
Gene Aliases: ATP6A
UniProt ID: (Human) P20648
Entrez Gene ID: (Human) 495
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