Zitierungen und Referenzen (6)

Invitrogen™

Gateway™ pDEST™10 Vector

Um all Ihren Expressionsansprüche zu entsprechen, bietet Invitrogen modernste Gateway™-Zielvektoren für die Expression in E. coli-, Insekten-, Hefe- oder SäugetierzellenWeitere Informationen

Katalognummer	Menge
11806015	6μ g

Katalognummer 11806015

Preis (EUR)

1.450,00

Menge:

Preis (EUR)

1.450,00

Um all Ihren Expressionsansprüche zu entsprechen, bietet Invitrogen modernste Gateway™-Zielvektoren für die Expression in E. coli-, Insekten-, Hefe- oder Säugetierzellen sowie für die Produktion von nativen Proteinen bzw. von Fusionsproteinen am N- bzw. C-Terminal. Alle Gateway™-Zielvektoren verfügen über attR-Stellen für die Rekombination mit jedem von attL-flankierten Fragment, unabhängig davon, ob es sich um einen Entry-Klon oder einen Ultimate™ RF-Klon handelt. In der folgenden Tabelle sind die zahlreichen verfügbaren Zielvektoren aufgeführt.

Zusätzliches Material erforderlich, separat erhältlich: Gateway™ Entry-Klon, Gateway™ LR Clonase™ Enzymgemisch und Reaktionspuffer.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

SpaltungTeV-Protease-Erkennungsstelle

ProdukttypGateway System Destinationsexpressionsvektor

Menge6μ g

VektorpDEST, Gateway Baculovirus-Vektoren

KlonierungsmethodeGateway™

ProduktlinieGateway

PromoterPolyhedrin

ProteinmarkierungHis-Tag (6x)

Unit SizeEach

Inhalt und Lagerung

Alle Zielvektoren werden lyophilisiert und superspiralisiert geliefert.

Häufig gestellte Fragen (FAQ)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Gateway™ pDEST™10 Vector FAQs

Zitierungen und Referenzen (6)

Zitierungen und Referenzen

Abstract

Munc13-4 is a GTP-Rab27-binding protein regulating dense core granule secretion in platelets.

Authors:Shirakawa R, Higashi T, Tabuchi A, Yoshioka A, Nishioka H, Fukuda M, Kita T, Horiuchi H,

Journal:J Biol Chem

PubMed ID:14699162

'Platelets store self-agonists such as ADP and serotonin in dense core granules. Although exocytosis of these granules is crucial for hemostasis and thrombosis, the underlying mechanism is not fully understood. Here, we show that incubation of permeabilized platelets with unprenylated active mutant Rab27A-Q78L, wild type Rab27A, and Rab27B inhibited the ... More

SCAP ligands are potent new lipid-lowering drugs.

Authors: Grand-Perret T; Bouillot A; Perrot A; Commans S; Walker M; Issandou M;

Journal:Nat Med

PubMed ID:11726962

'Upregulation of low-density lipoprotein receptor (LDLr) is a key mechanism to control elevated plasma LDL-cholesterol levels. Here we identify a new class of compounds that directly binds to the sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP). We show that a 14C-labeled, photo-activatable analog specifically labeled both SCAP and a ... More

Enhanced production of green fluorescent fusion proteins in a baculovirus expression system by addition of secretion signal.

Authors:Katagiri Y, Ingham KC.

Journal:Biotechniques

PubMed ID:12139250

DNA cloning using in vitro site-specific recombination.

Authors: Hartley J L; Temple G F; Brasch M A;

Journal:Genome Res

PubMed ID:11076863

As a result of numerous genome sequencing projects, large numbers of candidate open reading frames are being identified, many of which have no known function. Analysis of these genes typically involves the transfer of DNA segments into a variety of vector backgrounds for protein expression and functional analysis. We describe ... More

Role of the G protein gamma subunit in beta gamma complex modulation of phospholipase Cbeta function.

Authors: Akgoz Muslum; Azpiazu Inaki; Kalyanaraman Vani; Gautam N;

Journal:J Biol Chem

PubMed ID:11914377

The G protein betagamma complex regulates a wide range of effectors, including the phospholipase C isozymes (PLCbetas). Different domains on the beta subunit are known to contact phospholipase Cbeta and affect its regulation. In contrast, the role of the gamma subunit in Gbetagamma modulation of PLCbeta function is not known. ... More

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