Thermo Scientific™

PageRuler™ ungefärbte Proteinleiter

Name: PageRuler™ ungefärbte Proteinleiter
SKU: 26614
Price: 107.00 EUR

Die Thermo Scientific PageRuler ungefärbte Proteinleiter ist eine Mischung aus 14 rekombinanten, hochaufgereinigten, ungefärbten Proteinen (10 bis 200 kDa) zurWeitere Informationen

Ansicht ändern

Katalognummer	Menge
26614	2 x 250 μL
26614X4	8 x 250 μL

2 Optionen

Katalognummer 26614

Preis (EUR)

107,00

Each

Menge:

2 x 250 μL

Großbestellung oder individuelle Größe anfordern

Preis (EUR)

107,00

Each

Die Thermo Scientific PageRuler ungefärbte Proteinleiter ist eine Mischung aus 14 rekombinanten, hochaufgereinigten, ungefärbten Proteinen (10 bis 200 kDa) zur Verwendung als Größenstandards in der Proteinelektrophorese (SDS-PAGE) und zum Western Blotting. Die Proteinleiter wird in einem gebrauchsfertigen Format geliefert für die direkte Auftragung auf Gelen. Vor der Verwendung muss kein Probenpuffer erwärmt, reduziert oder hinzugefügt werden.

Alle anderen Proteinstandards und -leitern anzeigen und vergleichen ›

Produktmerkmale
• Referenzbanden – 50 kDa-Bande ist stärker als die anderen und erleichtert die Orientierung
• Getaggt – die Leiterproteine enthalten eine integrierte Strep-tag™ II-Sequenz und können mit Strep-Tactin™ Konjugaten oder einem Antikörper gegen die Strep-tag™ II-Sequenz auf Western Blots nachgewiesen werden

Anwendungen
• Genaue Größenbestimmung von Proteinen auf SDS-Polyacrylamid-Gelen und Western Blots

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

GelkompatibilitätBolt™ Bis-Tris Plus Gele, Novex™ Tricin-Gele, Novex™ Tris-Glycin-Gele, NuPAGE™ Bis-Tris-Gele, NuPAGE™ Tris-Acetat-Gele, SDS-PAGE-Gele

Molekulargewicht200, 150, 120, 100, 85, 70, 60, 50, 40, 30, 25, 20, 15, 10 kDa

Menge2 x 250 μL

Sofort einsatzbereitJa

VersandbedingungZugelassen für den Versand auf Nass- oder Trockeneis

Number of Markers14

ProduktliniePageRuler

ProdukttypProteinleiter

Größenbereich10 bis 200 kDa

Stain TypeUngefärbt

System TypeWestern Blotting, SDS-PAGE

Unit SizeEach

Inhalt und Lagerung

Inhalt: zwei 250 μL-Fläschchen, Proteine zu je 0,02 bis 0,05 mg/ml

Lagerpuffer: 62,5 mmol Tris-H₃PO₄ (pH 7,5 bei 25 °C), 1 mmol EDTA, 2 % SDS, 10 mmol DTT, 1 mmol NaN₃, 0,01 % Bromophenolblau und 33 % Glycerin

Lagerung: Nach Erhalt bei -20 °C lagern

Häufig gestellte Fragen (FAQ)

The upper bands of the ladder are missing. What could be the reason?

The upper bands of the ladder may be degraded by proteases. Ladder, gel, buffer, pipettes, pipette tips, or equipment can be contaminated by proteases during usage. A general recommendation would be to avoid working with proteases in the same room. We would recommend preparing fresh solutions, cleaning the equipment, and using clean pipettes and tips. If the ladder itself is contaminated, please use a new tube of the ladder.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Some additional bands or smears were observed on the gel when using a PageRuler unstained ladder. What may have caused this?

Additional bands can appear due to dithiothreitol (DTT) oxidation in the storage buffer. Please add newly prepared DTT solution to the final concentration of 100 mM and boil for 5 min at 95 degrees C. This should solve the issue. Addition of DTT is NOT recommended for prestained protein ladders, since too high a concentration of reducing agents can cause protein destaining.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do the proteins in Thermo Scientific protein ladders have a His-Tag or would otherwise react with an anti-His-Tag antibody?

No, proteins in Thermo Scientific protein ladders are not His tagged. However, non-specific interaction between the ladder proteins and primary or secondary antibodies is possible and some His-Tag detection systems, such as Thermo Scientific 6xHis Protein Tag Stain Reagent Kit, show non-specific interaction. The protein ladder bands are more readily detected when using high antibody concentrations. The non-specific cross-reactivity is difficult to predict, it often has a different pattern dependent on the antibodies used in each individual experiment. The most general way to handle this problem would be to use lower concentrations of antibodies and to use lower amount of protein ladders. It may also be useful to leave one empty well between the ladder and the sample to overcome a possible leakage of the signal to the nearby sample lane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can Thermo Scientific protein ladders be detected by Strep-Tactin conjugates?

PageRuler Unstained protein ladders can be detected directly on Western blots by using Strep-Tactin conjugates or an antibody against the Strep-tag II sequence. All PageRuler and Spectra ladder proteins contain an integral Strep-tag II sequence, however the prestained ladders cannot be detected by Strep-Tactin conjugates.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Why is non-specific binding detected after Western blot?

Protein ladder bands can sometimes be detected with chemiluminescent techniques due to non-specific interactions of ladder proteins with either primary or secondary antibodies (or with both). The ladder bands are only rarely detected by chromogenic substrates. The extremely high sensitivity of the chemiluminescent assays is needed to see the bands, so the actual degree of cross-reactivity is low. The non-specific cross-reactivity is difficult to predict, it often has a different pattern depending on the antibodies used. If antibodies recognize a linear epitope, the cross-reactivity may be due to sequence homology. If antibodies react with a denaturation-resistant conformational epitope it could be impossible to identify the exact reason for detected cross-reactivity. The most general way to handle this problem would be to use lower concentrations of antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all PageRuler™ ungefärbte Proteinleiter FAQs