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Citations & References (7)
Thermo Scientific™
EMCS (N-ε-malemidocaproyl-oxysuccinimide ester)
Thermo Scientific Pierce EMCS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of aRead more
| Catalog Number | Quantity |
|---|---|
| 22308 | 50 mg |
Catalog number 22308
Price (EUR)
339,00
Each
Quantity:
50 mg
Price (EUR)
339,00
Each
Thermo Scientific Pierce EMCS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length spacer arm (9.4 angstroms).
Features of EMCS:
• Reactive groups: NHS ester and maleimide
• Reactive towards: amino and sulfhydryl groups
• C6-homolog of aliphatic spacer series which includes AMAS, BMPS, GMBS and EMCS
• NHS ester end couples with primary amines at pH 7-9 to form stable amide bonds
• Maleimides react with -SH groups at pH 6.5-7.5, forming stable thioether linkages
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-EMCS
• Increased sphere of coupling vs. GMBS
• Short aliphatic spacer has low potential for eliciting an immune response
Product References:
Crosslinker Application Guide -- search for recent literature references for this product
Features of EMCS:
• Reactive groups: NHS ester and maleimide
• Reactive towards: amino and sulfhydryl groups
• C6-homolog of aliphatic spacer series which includes AMAS, BMPS, GMBS and EMCS
• NHS ester end couples with primary amines at pH 7-9 to form stable amide bonds
• Maleimides react with -SH groups at pH 6.5-7.5, forming stable thioether linkages
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-EMCS
• Increased sphere of coupling vs. GMBS
• Short aliphatic spacer has low potential for eliciting an immune response
Product References:
Crosslinker Application Guide -- search for recent literature references for this product
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell PermeabilityYes
DescriptionEMCS
FormPowder
Labeling MethodChemical Labeling
Molecular Weight (g/mol)308.29
PEGylatedNo
Product LinePierce
Quantity50 mg
Reactive MoietyMaleimide, NHS Ester
Shipping ConditionAmbient
SolubilityDMF, DMSO
Spacer Arm Length9.4 Å
Water SolubleNo
Chemical ReactivityAmine-Sulfhydryl
CleavableNo
Crosslinker TypeHeterobifunctional
FormatStandard
Product TypeCrosslinker
SpacerShort (<10 Å)
Unit SizeEach
Contents & Storage
Upon receipt store desiccated at 4°C.
Frequently asked questions (FAQs)
Can you provide the shelf-life for EMCS (N-ε-malemidocaproyl-oxysuccinimide ester)?
Citations & References (7)
Citations & References
Abstract
Mechanism of reversal of phospholamban inhibition of the cardiac Ca2+-ATPase by protein kinase A and by anti-phospholamban monoclonal antibody 2D12.
Journal:J Biol Chem
PubMed ID:17548345
'Our model of phospholamban (PLB) regulation of the cardiac Ca(2+)-ATPase in sarcoplasmic reticulum (SERCA2a) states that PLB binds to the Ca(2+)-free, E2 conformation of SERCA2a and blocks it from transitioning from E2 to E1, the Ca(2+)-bound state. PLB and Ca(2+) binding to SERCA2a are mutually exclusive, and PLB inhibition of
Miniature single-particle immunoassay for prostate-specific antigen in serum using recombinant Fab fragments.
Journal:Clin Chem
PubMed ID:11067810
Quantitative, miniaturized nucleic acid assays and immunoassays can be developed with single microparticles, microfluorometric detection, and intrinsically fluorescent lanthanide chelates in a multiple assay format to decrease reagent consumption, cost, and assay time. We used recombinant Fab fragments to capture and detect free and total prostate-specific antigen (PSA) from serum
Characterizing phospholamban to sarco(endo)plasmic reticulum Ca2+-ATPase 2a (SERCA2a) protein binding interactions in human cardiac sarcoplasmic reticulum vesicles using chemical cross-linking.
Journal:J Biol Chem
PubMed ID:22247554
Chemical cross-linking was used to study protein binding interactions between native phospholamban (PLB) and SERCA2a in sarcoplasmic reticulum (SR) vesicles prepared from normal and failed human hearts. Lys(27) of PLB was cross-linked to the Ca(2+) pump at the cytoplasmic extension of M4 (at or near Lys(328)) with the homobifunctional cross-linker,
PNA microarrays for hybridisation of unlabelled DNA samples.
Journal:Nucleic Acids Res
PubMed ID:14500847
Several strategies have been developed for the production of peptide nucleic acid (PNA) microarrays by parallel probe synthesis and selective coupling of full-length molecules. Such microarrays were used for direct detection of the hybridisation of unlabelled DNA by time-of-flight secondary ion mass spectrometry. PNAs were synthesised by an automated process
Role of leucine 31 of phospholamban in structural and functional interactions with the Ca2+ pump of cardiac sarcoplasmic reticulum.
Journal:J Biol Chem
PubMed ID:15644311
The ability of two loss-of-function mutants, L31A and L31C, of phospholamban (PLB) to bind to and inhibit the Ca(2+) pump of cardiac sarcoplasmic reticulum (SERCA2a) was investigated using a molecular cross-linking approach. Leu(31) of PLB, located at the cytoplasmic membrane boundary, is a critical amino acid shown previously to be