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Alternative Product: Try
PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Maxima SYBR Green qPCR Master Mix and added additional capabilities for your gene expression analysis.
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Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start
Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
Maxima Hot Start
Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers (
see Resources for a compatibility chart).
Highlights•
Specificity—
Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
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Sensitivity—detects low copy number targets
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Wide linear range—accurate quantification across 9 orders of magnitude
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Reproducibility and convenience—ready-to-use 2x master mix
Applications• Gene expression
• siRNA validation
• Genotyping
• Pathogen detection
IncludesMaxima SYBR Green/ROX qPCR Master Mix (2x) includes Maxima Hot Start Taq DNA Polymerase and
dNTPs (also
dUTP) in an optimized PCR buffer supplemented with ROX passive reference dye. Supplied with nuclease-free water.
Related ProductsMaxima SYBR Green/ROX qPCR Master Mix (2X)Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vialFor Research Use Only. Not for use in diagnostic procedures.