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Supplément B-27™ (50X), sans vitamine A
Citations et références (2)
Gibco™

Supplément B-27™ (50X), sans vitamine A

Une variation du supplément de culture cellulaire neuronale le plus cité, le supplément B-27 moins la vitamine A est uneAfficher plus
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RéférenceQuantité
1258701010 mL
12587001100 mL
Référence 12587010
Prix (EUR)
193,00
Each
Quantité:
10 mL
Prix (EUR)
193,00
Each
Une variation du supplément de culture cellulaire neuronale le plus cité, le supplément B-27 moins la vitamine A est une version personnalisée du supplément B-27 original sans vitamine A (acétate de rétinyle). Le supplément B-27 moins la vitamine A est un supplément sans sérum idéal pour la culture de cellules progénitrices neurales et de cellules souches, sous forme de neurosphères en suspension ou de culture monocouche adhérente, sans induire de différenciation. Le supplément B-27 moins la vitamine A est fourni sous forme de liquide 50X et doit être utilisé avec un milieu Neurobasal ou un milieu Neurobasal-A pour la culture de cellules neuronales sans nécessiter de couche nourricière d’astrocytes.

Le supplément B-27 convient à la plupart des applications relatives aux cellules neuronales. Pour d’autres applications, nous fournissons des formulations alternatives de suppléments B-27 et de milieux Neurobasal.

Pour les protocoles établis à l’aide du supplément B-27, consultez notre manuel sur le protocole de neurobiologie Gibco.

Consultez les publications à l’aide des produits de neurobiologie Gibco chez notre Gibco CellCite.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Type de celluleCellules progénitrices neurales
Concentration50X
Type de cultureCulture de cellules de mammifères
Gamme de produitsB-27
Quantité10 mL
Niveau de sérumSans sérum
Durée de conservation12 mois
Conditions d’expéditionGlace carbonique
SourceSans sérum
Testé pourEndotoxine, performances, stérilité, mycoplasmes
ClassificationSans sérum
FormeLiquide
Type de produitSupplément de culture cellulaire
StérilitéStérile
Unit SizeEach
Contenu et stockage
Conserver au congélateur (-5 à -20°C) à l’abri de la lumière.

Foire aux questions (FAQ)

Can B-27 supplement be filtered?

Yes, the protein content in this product is high enough so filtering through a low protein binding filter as a 50X or 1X in solution should not be a problem.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can Neurobasal Medium alone or Neurobasal Medium supplemented with B-27 supplement be frozen to extend product shelf life?

No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What supplements should I use with Neurobasal or Neurobasal-A medium?

Neurobasal and Neurobasal-A media (for postnatal and adult neurons) allow for long-term maintenance of neuronal cells without the need for an astrocyte feeder layer. These media should be supplemented with either serum or a serum-free supplement, plus 0.5mM L-glutamine. B-27 supplement is a serum-free supplement that comes as a 50X concentrate in a 10ml volume. This is enough supplement for 500ml of media. Fetal, postnatal, and adult neural cultures can be grown in the appropriate Neurobasal medium supplemented with B-27 supplement .

We also have two other supplements. One is called G-5 and is for growth and expression of glial cells (normal and tumor) of astrocytic phenotype. This comes in a 1ml size, at a 100X concentration. The other supplement is called N-2 and is for growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. This comes in a 5ml size, at a 100X concentration.

For more information on Neurobasal media, search "Neurobasal" from our website home page.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer B-27 supplements with and without insulin?

Yes, we do offer B-27 Supplement, minus insulin (Cat. Nos. A1895601 and A1895602). All our other B-27 supplements do contain insulin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I thaw B-27 Supplements in a 37 degrees C water bath or incubator?

We do not recommend thawing at 37 degrees C. Best results are observed upon thawing at room temperature for approximately 2 hrs or overnight at 2-8 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Supplément B-27™ (50X), sans vitamine A FAQs

Citations et références (2)

Citations et références
Abstract
Patient-derived glioblastoma organoids as real-time avatars for assessing responses to clinical CAR-T cell therapy
Authors:Meghan Logun, Xin Wang, Yusha Sun, Stephen J Bagley, Nannan Li, Arati Desai, Daniel Y Zhang, MacLean P Nasrallah, Emily Ling-Lin Pai, Bike Su Oner, Gabriela Plesa, Donald Siegel, Zev A Binder, Guo-Li Ming, Hongjun Song, Donald M O'Rourke
Journal:Cell Stem Cell
PubMed ID:39657679
Patient-derived tumor organoids have been leveraged for disease modeling and preclinical studies but rarely applied in real time to aid with interpretation of patient treatment responses in clinics. We recently demonstrated early efficacy signals in a first-in-human, phase 1 study of dual-targeting chimeric antigen receptor (CAR)-T cells (EGFR-IL13Rα2 CAR-T cells) ... More
Type I astrocytes and microglia induce a cytokine response in an encephalitic murine coronavirus infection.
Authors:Lavi E, Cong L
Journal:Exp Mol Pathol
PubMed ID:32454103
'The pathogenesis of viral infections involves an immune response by cytokines, causing a deleterious effect on organ function, in addition to tissue destruction due to viral replication. Clinical symptoms and laboratory findings of the human coronavirus disease COVID-19, caused by the novel coronavirus SARS CoV-2, indicate cytokine involvement. Our laboratory ... More