Invitrogen™

Cartes iBind™ Flex

Name: Cartes iBind™ Flex
SKU: SLF2010X4
Price: 966.00 EUR

Les cartes iBind Flex sont utilisées avec le dispositif Western iBind Flex qui est un dispositif de traitement Western automatiséAfficher plus

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Référence	Type	Quantité
SLF2010X4	Carte	40 cartes (10 x 4)
SLF2010	Carte	10 cartes

2 options

Référence SLF2010X4

Prix (EUR)

966,00

10 cards,40 cards

Type:

Carte

Quantité:

40 cartes (10 x 4)

Prix (EUR)

966,00

10 cards,40 cards

Les cartes iBind Flex sont utilisées avec le dispositif Western iBind Flex qui est un dispositif de traitement Western automatisé qui exécute chaque étape du flux de travaux Western, du blocage aux lavages aux incubations d’anticorps. Chaque solution est émise par les puits du dispositif iBind Flex vers la carte iBind Flex au moyen d’un flux latéral séquentiel (SLF), les solutions sont ensuite dirigées vers la région de pile de la carte. La matrice en fibre de verre de la carte permet un flux homogène et constant des solutions en direction de la membrane, augmentant ainsi l’interaction antigène-anticorps.

En savoir plus sur les systèmes iBind Western ›

Les cartes iBind Flex associées aux Dispositif Western iBind Flex et kit de solution iBind Flex ou kit de solution pour détection par fluorescence (FD) iBind Flex apportent automatisation et simplicité à votre protocole Western. Chaque carte iBind Flex peut traiter jusqu’à un transfert midi, deux transferts mini ou six transferts de bandes à coupe verticale.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Méthode de détectionChromogène, chimiluminescent, fluorescent

Compatibilité avec les membranesNitrate de cellulose, PVDF

Quantité40 cartes (10 x 4)

Conditions d’expéditionTempérature ambiante

Cadence1 transfert midi, 6 transferts de barrettes à coupe verticale, 2 transferts mini par carte

Capacité1 transfert midi, 6 transferts de barrettes à coupe verticale, 2 transferts mini

À utiliser avec (application)Transfert Western

À utiliser avec (équipement)Dispositif Western iBind Flex

Gamme de produitsiBind Flex

Running Time∼2,5 heures

TypeCarte

Unit Size10 cards,40 cards

Contenu et stockage

Conserver à température ambiante.

Foire aux questions (FAQ)

Can I use conjugated primary antibodies with the iBind Flex Western Device?

Yes. When using a conjugated primary antibody and no secondary antibody, add the diluted primary antibody to well 1 and replace diluted secondary antibody (well 3) with 1X iBind Flex/iBind Flex FD Solution. Alternatively, for faster time to results, add the diluted primary antibody to well 1, leave wells 2 and 3 empty, and add 1X iBind Flex/ iBind Flex FD Solution to well 4. This procedure will save time (~60 min) but may result in higher background.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What procedure should I follow when stripping and reprobing my blot processed using the iBind Western Device or iBind Flex Western Device?

To strip and reprobe your blot processed using the iBind Western Device or iBind Flex Western Device, first strip the blot outside the system using your preferred protocol. After the stripping protocol, wash the blot in distilled water for 5 min. Reblocking is unnecessary as the iBind Solution will perform this step during reprobing. Finally, reprobe with new antibodies using the iBind Western Device or iBind Flex Western Device, starting with the membrane preparation step.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I multiplex antibodies using the iBind Western Device or iBind Flex Western Device?

Yes, for each midi blot, mini blot, or vertically cut strip, prepare appropriate primary antibodies together in a single tube of iBind FD/ iBind Flex FD Solution, Prepare appropriate secondary antibodies together in a different tube of iBind FD/ iBind Flex FD Solution. Do not mix primary and secondary antibodies prior to loading in the device. All primary and secondary antibodies should be diluted according to the recommendations outlined in the user manual or quick reference card. When detecting several targets of the same species, the same concentration of secondary antibody can be used as when detecting one target. There is no need to increase the concentration for each additional target. Load multiplexed antibodies into the device as normal.
Caution: Consider cross-reactivity of secondary antibodies when multiplexing (e.g., rabbit anti-goat IgG and goat anti-mouse IgG are likely to cross-react).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the iBind Western Device or iBind Flex Western Device with Alexa Fluor Plus conjugated secondary antibodies?

Yes, both the iBind Western Device and iBind Flex Western Device work well with Alexa Fluor Plus conjugated secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

With the iBind Western Device and iBind Flex Western Device, how do I know if I am rolling the membrane firmly enough?

When firmly rolling, the iBind/iBind Flex Card will slightly dip in the membrane area, causing the top and bottom of the card to lift. See the iBind/iBind Flex user manual for additional details.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all Cartes iBind™ Flex FAQs