Invitrogen™

iBind™ Flex Fluorescent Detection (FD) Solution Kit

La solution de détection par fluorescence (FD) iBind Flex est compatible avec la détection par fluorescence (par exemple double longueurAfficher plus

Référence	Quantité
SLF2019	1 kit

Référence SLF2019

Prix (EUR)

181,00

1 kit

Quantité:

1 kit

Prix (EUR)

181,00

1 kit

La solution de détection par fluorescence (FD) iBind Flex est compatible avec la détection par fluorescence (par exemple double longueur d’onde IR) et sert de solution combinée de blocage, de lavage et de diluant d’anticorps pour traiter jusqu’à un transfert midi, deux transferts mini ou six transferts de bandes découpés verticalement lorsqu’elle est utilisée avec le dispositif iBind Flex Western et les cartes iBind Flex. Chaque kit contient suffisamment de réactifs pour 10 transferts midi ou 20 transferts mini.

En savoir plus sur les systèmes iBind Western ›

Système iBind Flex Western
Le système iBind Flex Western assure un traitement automatique des transferts Western et peut prendre en charge différents formats de transfert, y compris les bandelettes midi, mini et coupées à la verticale. Suivant une technologie d’écoulement latéral séquentiel (SLF), toutes les étapes de blocage, d’incubation des anticorps et de lavage au cours de l’immunodétection ne nécessitent aucune intervention manuelle. Par rapport aux méthodes manuelles, le système iBind Flex Western nécessite moins d’anticorps primaires, permettant ainsi d’obtenir des résultats reproductibles. Le système est compatible avec les protocoles de détection en aval.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

À utiliser avec (équipement)Dispositif iBind Flex Western

Contenus des kitsTampon iBind™ Flex FD 5X, Additif iBind™ Flex 100X, SDS iBind™ Flex FD 10 %

Nbre de réactions10 transferts midi ou 20 transferts mini

Quantité1 kit

Conditions d’expéditionHomologué pour des expéditions à température ambiante ou sur glace mouillée

Méthode de détectionFluorescence

Membrane CompatibilityPVDF, nitrocellulose

Gamme de produitsiBind

Type de produitKit de solutions Flex

Unit Size1 kit

Contenu et stockage

• Tampon iBind Flex FD 5X
• Additif iBind Flex 100X
• SDS à 10 % iBind Flex FD

Conserver entre 2 et 8°C.

Foire aux questions (FAQ)

Can I use conjugated primary antibodies with the iBind Flex Western Device?

Yes. When using a conjugated primary antibody and no secondary antibody, add the diluted primary antibody to well 1 and replace diluted secondary antibody (well 3) with 1X iBind Flex/iBind Flex FD Solution. Alternatively, for faster time to results, add the diluted primary antibody to well 1, leave wells 2 and 3 empty, and add 1X iBind Flex/ iBind Flex FD Solution to well 4. This procedure will save time (~60 min) but may result in higher background.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What procedure should I follow when stripping and reprobing my blot processed using the iBind Western Device or iBind Flex Western Device?

To strip and reprobe your blot processed using the iBind Western Device or iBind Flex Western Device, first strip the blot outside the system using your preferred protocol. After the stripping protocol, wash the blot in distilled water for 5 min. Reblocking is unnecessary as the iBind Solution will perform this step during reprobing. Finally, reprobe with new antibodies using the iBind Western Device or iBind Flex Western Device, starting with the membrane preparation step.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I multiplex antibodies using the iBind Western Device or iBind Flex Western Device?

Yes, for each midi blot, mini blot, or vertically cut strip, prepare appropriate primary antibodies together in a single tube of iBind FD/ iBind Flex FD Solution, Prepare appropriate secondary antibodies together in a different tube of iBind FD/ iBind Flex FD Solution. Do not mix primary and secondary antibodies prior to loading in the device. All primary and secondary antibodies should be diluted according to the recommendations outlined in the user manual or quick reference card. When detecting several targets of the same species, the same concentration of secondary antibody can be used as when detecting one target. There is no need to increase the concentration for each additional target. Load multiplexed antibodies into the device as normal.
Caution: Consider cross-reactivity of secondary antibodies when multiplexing (e.g., rabbit anti-goat IgG and goat anti-mouse IgG are likely to cross-react).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the iBind Western Device or iBind Flex Western Device with Alexa Fluor Plus conjugated secondary antibodies?

Yes, both the iBind Western Device and iBind Flex Western Device work well with Alexa Fluor Plus conjugated secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

With the iBind Western Device and iBind Flex Western Device, how do I know if I am rolling the membrane firmly enough?

When firmly rolling, the iBind/iBind Flex Card will slightly dip in the membrane area, causing the top and bottom of the card to lift. See the iBind/iBind Flex user manual for additional details.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all iBind™ Flex Fluorescent Detection (FD) Solution Kit FAQs