EMCS (N-ε-malemidocaproyl-oxysuccinimide ester)

Thermo Scientific Pierce EMCS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length spacer arm (9.4 angstroms).

Features of EMCS:

• Reactive groups: NHS ester and maleimide
• Reactive towards: amino and sulfhydryl groups
• C6-homolog of aliphatic spacer series which includes AMAS, BMPS, GMBS and EMCS
• NHS ester end couples with primary amines at pH 7-9 to form stable amide bonds
• Maleimides react with -SH groups at pH 6.5-7.5, forming stable thioether linkages
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-EMCS
• Increased sphere of coupling vs. GMBS
• Short aliphatic spacer has low potential for eliciting an immune response

Product References:
Crosslinker Application Guide -- search for recent literature references for this product