Biocytin Alexa Fluor™ 488 (Alexa Fluor™ 488 Biocytin, Disodium Salt)

Citations & References (9)

Invitrogen™

Biocytin Alexa Fluor™ 488 (Alexa Fluor™ 488 Biocytin, Disodium Salt)

The cell-impermeant, fixable, polar tracer Alexa Fluor™ 488 biocytin combines the green-fluorescent Alexa Fluor™ 488 fluorophore with biotin and anRead more

Catalog Number	Quantity
A12924	250 μg

Catalog number A12924

Price (JPY)

67,400

Quantity:

250 μg

The cell-impermeant, fixable, polar tracer Alexa Fluor™ 488 biocytin combines the green-fluorescent Alexa Fluor™ 488 fluorophore with biotin and an aldehyde-fixable primary amine. Polar tracers are commonly used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis. This water-soluble tracer can be introduced into cells by whole-cell patch clamping, iontophoresis, osmotic lysis of pinocytic vesicles or comparable methods. The biotin allows for detection and subsequent amplification with enzyme-modified streptavidins including horseradish (HRP) and alkaline phosphatase (AP) that can be used with TSA and ELF technologies, respectively.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or DyeAlexa Fluor Dyes

Quantity250 μg

Shipping ConditionRoom Temperature

Product LineAlexa Fluor

Unit SizeEach

Contents & Storage

Store at room temperature and protect from light.

Frequently asked questions (FAQs)

I injected a fluorescent tracer, but cannot detect it after tissue is fixed and sectioned. What am I doing wrong?

Confirm that the tracer you are using crosslinks to proteins or has a primary amine for fixation-either a hydrazide, lysine fixable dextran, or a protein conjugate.
Use aldehyde-based fixatives to cross link the amines on the tracer.
Inject a larger amount or higher concentration of the tracer. Tracers are generally injected at 1-20% concentrations (10 mg/mL or higher).
Confirm that you are using the correct fluorescent filter for detection. You can perform a spot test by pipetting a small amount of the undiluted stock solution of the tracer onto a slide, then view under the filter you are using on your microscope. This will confirm if the tracer fluorescence can be detected and the fluorescent microscope filter is working properly.
Review tissue fixation and handling procedures to confirm if any reagents or processing procedures could be affecting the tracer.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have labeled my neurons with an Alexa Fluor conjugated biocytin to look at transport but I wanted to examine only retrograde transport and biocytin appears to be moving retrograde and anterograde. What should I do?

Observing both types of transport is typical for biocytin. The conjugated cholera toxin subunit B products have been observed to travel only retrogradely.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you have a neuronal tracer similar to Lucifer Yellow but in another fluorescent color?

Lucifer Yellow CH is a hydrazide, so any of our Alexa Fluor or fluorescent hydrazides could potentially be used. A listing of them can be found here. (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing/hydrazides-biocytins.html#prd)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (9)

Citations & References

Abstract

Deconvolving single-molecule intensity distributions for quantitative microscopy measurements.

Authors:Mutch SA, Fujimoto BS, Kuyper CL, Kuo JS, Bajjalieh SM, Chiu DT,

Journal:Biophys J

PubMed ID:17259276

'In fluorescence microscopy, images often contain puncta in which the fluorescent molecules are spatially clustered. This article describes a method that uses single-molecule intensity distributions to deconvolve the number of fluorophores present in fluorescent puncta as a way to "count" protein number. This method requires a determination of the correct ... More

Hebbian LTP in feed-forward inhibitory interneurons and the temporal fidelity of input discrimination.

Authors:Lamsa K, Heeroma JH, Kullmann DM

Journal:Nat Neurosci

PubMed ID:15937481

'Cortical information processing requires a delicate balance of excitatory and inhibitory signaling. How is this balance preserved during hippocampal memory encoding, which involves NMDA receptor-dependent long term potentiation (LTP)? This form of LTP occurs at synapses between pyramidal neurons but has not been detected in feed-forward inhibitory interneurons. We show ... More

Evolutionary origins for social vocalization in a vertebrate hindbrain-spinal compartment.

Authors:Bass AH, Gilland EH, Baker R,

Journal:Science

PubMed ID:18635807

'The macroevolutionary events leading to neural innovations for social communication, such as vocalization, are essentially unexplored. Many fish vocalize during female courtship and territorial defense, as do amphibians, birds, and mammals. Here, we map the neural circuitry for vocalization in larval fish and show that the vocal network develops in ... More

Electrophysiological properties of morphologically-identified medial vestibular nucleus neurons projecting to the abducens nucleus in the chick embryo.

Authors:Gottesman-Davis A, Shao M, Hirsch JC, Peusner KD,

Journal:Neuroscience

PubMed ID:20971163

'Neurons in the medial vestibular nucleus (MVN) show a wide range of axonal projection pathways, intrinsic firing properties, and responses to head movements. To determine whether MVN neurons participating in the vestibulocular reflexes (VOR) have distinctive electrophysiological properties related to their output pathways, a new preparation was devised using transverse ... More

The styryl dye FM1-43 suppresses odorant responses in a subset of olfactory neurons by blocking cyclic nucleotide-gated (CNG) channels.

Authors:Breunig E, Kludt E, Czesnik D, Schild D,

Journal:J Biol Chem

PubMed ID:21646359

'Many olfactory receptor neurons use a cAMP-dependent transduction mechanism to transduce odorants into depolarizations. This signaling cascade is characterized by a sequence of two currents: a cation current through cyclic nucleotide-gated channels followed by a chloride current through calcium-activated chloride channels. To date, it is not possible to interfere with ... More

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