ReproSeq™ PGS 318 View Kit, with chips

The ReproSeq™ PGS View kits enable a rapid benchtop next-generation sequencing (NGS) workflow for aneuploidy analysis. When used with theRead more

Catalog Number	Quantity
A32250	192 samples

Catalog number A32250

Price (JPY)

Quantity:

192 samples

The ReproSeq™ PGS View kits enable a rapid benchtop next-generation sequencing (NGS) workflow for aneuploidy analysis. When used with the Ion PGM™ System, they allow for rapid and affordable detection of aneuploidies across all 24 chromosomes (22 autosomes and the X and Y chromosomes) for labs interested in rapidly and reliably assessing pre-implantation embryos for research. Using as little as 6 pg of DNA from a single cell, ReproSeq PGS View kits enable accurate detection of whole chromosome and chromosome arm events (≥48 Mb) in as little as 8 hours.* The Ion PGM sequencing system provides throughput flexibility, enabling the cost-effective screening of 2 to 24 samples and minimizing the need to batch samples.

*Turnaround time for the ReproSeq PGS View workflow is as little as 8 hours with the Ion 314™ Chip and as little as 10 hours with the Ion 318™ Chip.

Cost-effective analysis workflow with sample throughput flexibility
The ReproSeq PGS View kits and the Ion PGM System deliver scalability and flexibility for various levels of sample throughput. Choose the one that fits your throughput needs from a selection of 3 sequencing chips for the processing of 2 to 24 samples, thereby minimizing the need for batching to achieve cost efficiency.

Rapid turnaround for time-sensitive samples
Leveraging the speed of semiconductor sequencing and template preparation by isothermal amplification, the ReproSeq PGS View kits offer a fast benchtop NGS workflow for aneuploidy analysis. Go from single cells isolated from a pre-implantation embryo to aneuploidy detection in as little as 8 hours when screening up to 2 samples or as little as 10 hours with up to 24 samples. This rapid turnaround time enables fast identification of chromosomally normal embryos for research.

The ReproSeq™ PGS 318 View Kit, with chips, includes all reagents for whole genome amplification, library construction, template preparation, and sequencing using the Ion PGM System for processing a total of 192 samples. The kit contains:

• Ion SingleSeq™ Kit (24 reactions/kit)—allows for the extraction, amplification, and barcoding of genomic DNA, with completion in less than 4 hours (including hands-on time)
• Ion PGM™ Template IA 500 Kit (A24622, 8 reactions/kit)—enables the fastest template preparation (2 hours, including hands-on time) whereby DNA is clonally amplified onto a bead surface through a non-emulsion, isothermal reaction
• Ion PGM™ Hi-Q™ View Sequencing Kit (A30044, 8 reactions/kit at 200 bp)—offers our most advanced sequencing chemistry and is configured to support all 200 bp sequencing needs
• Ion PGM™ Wash 2 Bottle Kit (A25591)—contains conditioning solution and a Wash 2 bottle that may be used for up to 40 runs before replacement
• Ion 314/316/318™ Chip Kit v2 BC (4488150)—contains 8 barcoded chips per kit for sample tracking and sequencing runs using the Ion PGM System

Notes
• An Ion OneTouch™ ES (enrichment system) and Ion PGM™ Enrichment Beads (4478525) are required for use with this sequencing kit.
• This kit is only compatible with the Ion PGM System.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)Rapid Sequencing

For Use With (Equipment)Ion PGM™ System

Product TypePGS 318 View Kit With Chip

Quantity192 samples

Shipping ConditionApproved for shipment on Wet or Dry Ice

Throughput192 Samples

Product LineReproSeq

Starting MaterialDNA

Unit SizeEach

Contents & Storage

• Ion PGM Template IA Supplies 500 (store at room temperature)
• Ion PGM Template IA Reagents 500 (2–8°C)
• Ion PGM Template IA Reactions 500 (-5 to -30°C)
• Ion PGM Template IA Solutions 500 (room temperature)
• Ion SingleSeq Kit (-5 to -30°C)
• Ion PGM Seq Supplies (room temperature)
• Ion PGM Hi-Q View Seq Reagents (-5 to -30°C)
• Ion PGM Hi-Q View Seq Solutions (room temperature)
• Ion PGM Hi-Q Seq dNTP Kit (-5 to -30°C)
• Ion PGM Wash 2 Bottle Kit (room temperature)
• Ion 318™ Chip Kit v2 BC

Frequently asked questions (FAQs)

What are the catalog numbers for the Ion ReproSeq PGS View Kits?

Ion ReproSeq PGS 314 View Kit, without chips (16 samples): Cat. No. A32251
Ion ReproSeq PGS 316 View Kit, without chips (120 samples): Cat. No. A32252
Ion ReproSeq PGS 318 View Kit, without chips (192 samples): Cat. No. A32253
Ion ReproSeq PGS 314 View Kit, with chips (Up to 2 samples per chip, 8 chips included): Cat. No. A32243
Ion ReproSeq PGS 316 View Kit, with chips (Up to 15 samples per chip, 8 chips included): Cat. No. A32249
Ion ReproSeq PGS 318 View Kit, with chips (Up to 24 samples per chip, 8 chips included): Cat. No. A32250

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is included in the Ion PGM Wash 2 Bottle Kit (Cat. No. A25591)?

Here are the kit components:

Wash 2 Bottle w/label (2 L): 1 bottle
Wash 2 Bottle Conditioning Solution: 125 mL

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is included in the Ion PGM Hi‑Q View Sequencing Kit (Cat. No. A30044)?

Here are the kit components:

Ion PGM Sequencing Supplies (Part No. A25587)
- Wash 1 Bottle w/label (250 mL): 1 bottle
- Wash 3 Bottle w/label (250 mL): 1 bottle
- Ion PGM Reagent Bottle Sipper Tubes (blue): 16 tubes
- Ion PGM Wash Bottle Sipper Tubes (gray): 8 tubes for 250-mL bottles; 4 tubes for 2-L bottles
- Reagent Bottles w/labels (50 mL): 25 bottles

Ion PGM Hi-Q View Sequencing Reagents (Part No. A30043)
- Ion PGM Hi-Q View Sequencing Polymerase (yellow cap): 36 µL
- Ion PGM Hi-Q View Sequencing Primer (white cap): 144 µL
- Ion PGM Hi-Q View Control Ion PGM Ion Sphere Particles (clear cap): 60 µL

Ion PGM Hi-Q View Sequencing Solutions (Part No. A30275)
- Ion PGM Hi-Q View Sequencing W2 Solution: 4 x 125 mL
- Ion Cleaning Tablet: 4 tablets
- Annealing Buffer: 12 mL
- Ion PGM Hi-Q View Sequencing W3 Solution: 2 x 100 mL

Ion PGM Hi-Q Sequencing dNTPs (Part No. A25590)
- Ion PGM Hi-Q Sequencing dGTP (black cap): 80 µL
- Ion PGM Hi-Q Sequencing dCTP (blue cap): 80 µL
- Ion PGM Hi-Q Sequencing dATP (green cap): 80 µL
- Ion PGM Hi-Q Sequencing dTTP (red cap): 80 µL

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What are the components of the Ion PGM Template IA 500 Kit (Cat. No. A24622)?

Here are the kit components:

Ion PGM Template IA Supplies 500 (Part No. A24618)
- Ion OneTouch ES Supplies: 1 bag

Ion PGM Template IA Reagents 500 (Part No. A24619)
- Ion PGM Template IA Pellets 500: 4 foil pouches [1]
- Ion PGM Template IA ISP Dilution Buffer (yellow cap): 1 mL
- Ion PGM Template IA Start Solution (purple cap): 2 x 1200 µL

Ion PGM Template IA Reactions 500 (Part No. A24620)
- Ion PGM Template IA Ion Sphere Particles (orange cap): 86 µL
- Ion PGM Template IA Primer Mix S (black cap): 48 µL
- Ion PGM Template IA Primer Mix L (blue cap): 48 µL [2]
- Ion PGM Template IA Rehydration Buffer (white cap): 2 x 1.5 mL

Ion PGM Template IA Solutions (Part No. A24621)
- Ion PGM Template IA Stop Solution: 2.8 mL
- Ion PGM Template IA Recovery Solution: 7 mL
- Ion PGM Template IA Wash Solution: 4 mL
- MyOne Beads Wash Solution (green cap): 1.04 mL
- Neutralization Solution (red cap): 40 µL
- Tween Solution: 3.5 mL

Note: [1] One Ion PGM Template IA Pellet 500 per tube per pouch.
[2] Primer Mix L is intended for amplification of long templates and is not used with the Ion ReproSeq PGS View Kit

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is included in the Ion ReproSeq PGS View Kits?

Here are the kit components:

- Ion SingleSeq Kit (1, 5 or 8 boxes per Ion ReproSeq PGS View Kit, depending on the kit)
- Ion PGM Template IA 500 Kit (4 boxes/set, 1 set per Ion ReproSeq PGS View Kit)
- Ion PGM Hi-Q View Sequencing Kit (4 boxes/set, 1 set per Ion ReproSeq PGS View Kit)
- Ion PGM Wash 2 Bottle Kit (1 box per Ion ReproSeq PGS View Kit)
- Ion 314, Ion 316, or Ion 318 Chip Kit v2 BC (8 pack; Cat. Nos. A32243, A32249, A32250 only)

Note: The Ion SingleSeq Kit provides 24 unique Barcode Adapters and reagents to prepare 24 barcoded Ion SingleSeq libraries. The Ion PGM Template IA 500 Kit provides reagents and supplies to perform 4 isothermal amplification reactions. The Ion PGM Hi-Q View Sequencing Kit and Ion PGM Wash 2 Bottle Kit provide reagents and materials to perform 4 initializations and up to 12 sequencing runs on the Ion PGM Sequencer. Store the kit components as directed. See the Ion PGM Hi‑Q View Sequencing Kit User Guide (http://tools.thermofisher.com/content/sfs/manuals/MAN0014583_IonPGM_HiQ_View_Sequenc_UG.pdf) for more information.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

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