CultureOne™ Supplement (100X)

CultureOne Supplement enhances the differentiation of neural stem cells (NSCs) to neurons and can be used with primary rodent neurons or psc-derived neurons. A superior alternative to anti-mitotic agents like Ara-C or mitomycin C, CultureOne Supplement not only eliminates more than 75% of contaminating neural progenitor cells with minimal cell death, it also accelerates neuronal maturation. Using CultureOne Supplement in neuronal differentiation medium for two weeks yields more mature, functional neurons, reduces cell clumping, and allows maintenance of robust cultures for over a month.

CultureOne Supplement is a chemically-defined, serum-free supplement, provided as a 100X liquid reagent that can be easily added to any conventional neuronal differentiation medium. It should be used in place of other enrichment approaches such as Ara-C, mitomycin-C, fluorouracil (5-FU), or DAPT treatment.

Improved downstream assays

Analysis of NSC-derived neurons and primary rodent neurons can be difficult due to the presence of overgrown progenitor cells in the culture. These invasive cells complicate imaging and electrophysiology experiments and can affect the physiological relevance of gene expression assays. CultureOne Supplement efficiently controls the proliferation of both neural progenitor cells and glial cells, resulting in neurons of the highest purity and accelerating maturation and function.

Non-toxic proliferation control

Traditional methods to control proliferating cell populations involve treatments with anti-mitotic agents like cytosine arabinoside (Ara-C), which can be toxic to neurons. CultureOne Supplement controls proliferation without cytotoxicity. Cultures treated with CultureOne Supplement show better neurite outgrowth, less cell clumping, and reduced cell death compared to those treated with Ara-C or mitomycin C.

Accelerated maturation of differentiated neurons

NSC-derived neurons and primary rodent neurons treated with CultureOne Supplement express mature physiological markers faster and develop longer neurites than untreated cultures. These neurons also have significantly higher numbers of voltage-gated calcium ion channels, important for neural maturity and excitability.

Extended culture duration

CultureOne Supplement enables maintenance of enriched, differentiated neurons for five weeks or longer, unlike conventional methods that result in proliferating progenitor cells, causing clumping and peeling off the plate.

Combine CultureOne Supplement with B-27 Plus Neuronal Culture System and achieve a cleaner, more relevant neuronal cell population, without needing to resort to application of Ara-C, mitomycin-C, DAPT, or any other additions to complete your neuronal cell culture medium.