ELISA Buffer Kit

The ELISA Buffer Kit contains all the basic buffers necessary for use of Invitrogen Antibody Pairs or that are requiredRead more

Catalog Number	Quantity
CNB0011	10 plates

Catalog number CNB0011

Price (JPY)

115,200

Quantity:

10 plates

The ELISA Buffer Kit contains all the basic buffers necessary for use of Invitrogen Antibody Pairs or that are required to run an ELISA. The kit provides sufficient material for 10 ELISA plates.

Kit components:
• Coating Buffer A (10 mM phosphate buffer, pH 7.4)
• Coating Buffer B (50 mM carbonate buffer, pH 9.4)
• Assay Buffer (Blocking Buffer) (5X)
• Wash Buffer (25X)
• Stabilized TMB
• Stop Solution

View all available buffers and reagents for ELISA applications.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)ELISA

FormConcentrate

Shipping ConditionWet Ice

Quantity10 plates

Unit SizeEach

Contents & Storage

Coating Buffer A (100 mL): 1 bottle
Coating Buffer B (100 mL): 1 bottle
Assay Buffer (5x) (200 mL): 1 bottle
Wash Buffer (25x) (100 mL): 3 bottles
Stabilized Chromogen (25 mL): 4 bottles
Stop Solution (100 mL): 1 bottle. Store at 2°C to 8°C.

Frequently asked questions (FAQs)

How do I develop a sandwich ELISA using Cytosets?

Each CytoSets contains capture (coating) antibody, biotinlyated detection antibody, standard and Streptavidin-HRP. Other reagents required are listed in the CytoSets information sheet included with the kit and can be purshased from us separately (Antibody Pair Buffer kit CNB0011, 5x Assay Buffer DS98200, etc.). The information sheet also provides a specific procedure and illustrates an example standard curve which can be obtained when the specific procedure is followed. A general procedure is summarized here:

1) Coat the microplate with diluted capture (coating) antibody overnight at 2-8 degrees C; Wash the plate
2) Incubate standards or samples with the coated microplate; Wash the plate
3) Incubate diluted biotinlyated detection antibody with the plate; Wash the plate
4) Incubate Streptavidin-HRP with the plate for 15-45 minutes; Wash the plate
5) Incubate the plate with TMB substrate for 10-60 minutes and stop the reaction with Stop solution
6) Read microplate at 450 nm.

Investigators are advised to determine optimal buffer formulations, concentrations and incubation times for individual applications.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

Citations & References (2)

Citations & References

Abstract

Expression of innate immune response genes in liver and three types of adipose tissue in cloned pigs.

Authors:RÃ¸dgaard T, Skovgaard K, Stagsted J, Heegaard PM,

Journal:Cell Reprogram

PubMed ID:22928970

The pig has been proposed as a relevant model for human obesity-induced inflammation, and cloning may improve the applicability of this model. We tested the assumptions that cloning would reduce interindividual variation in gene expression of innate immune factors and that their expression would remain unaffected by the cloning process. ... More

Mechanisms of toxicity induced by SiO2 nanoparticles of in vitro human alveolar barrier: effects on cytokine production, oxidative stress induction, surfactant proteins A mRNA expression and nanoparticles uptake.

Authors:Farcal LR, Uboldi C, Mehn D, Giudetti G, Nativo P, Ponti J, Gilliland D, Rossi F, Bal-Price A,

Journal:Nanotoxicology

PubMed ID:22769972

'Abstract An in vitro human alveolar barrier established by the coculture of epithelial human cell line NCI-H441 with endothelial human cell line ISO-HAS1 was used to evaluate the effects of amorphous silicon dioxide nanoparticles (SiNPs), in the presence or absence of THP-1 cells (monocytes). SiNPs exposure induced production of proinflammatory ... More