The cell-impermeant Magnesium Green™, potassium salt exhibits a higher affinity for Mg2+ (Kd ∼1.0 mM) than mag-fura-2 (Kd ∼1.9 mM) or mag-indo-1 (Kd ∼2.7 mM). Upon binding Mg2+, Magnesium Green exhibits and increase in fluorescence emission intensity without a shift in wavelength with absorption/emission maxima ∼506/531 nm.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypeMagnesium Indicator
Quantity1 mg
Shipping ConditionRoom Temperature
For Use With (Equipment)Fluorescence Microscope, Microplate Reader
Product LineMagnesium Green
Product TypePentapotassium Salt
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.
Citations & References (27)
Citations & References
Abstract
Presynaptic Ca(2+) influx at the inhibitor of the crayfish neuromuscular junction: a photometric study at a high time resolution.
Authors:Vyshedskiy A, Lin JW
Journal:J Neurophysiol
PubMed ID:10634895
'Presynaptic calcium influx at the inhibitor of the crayfish neuromuscular junction was investigated by measuring fluorescence transients generated by calcium-sensitive dyes. This approach allowed us to correlate presynaptic calcium influx with transmitter release at a high time resolution. Systematic testing of the calcium indicators showed that only low-affinity dyes, with ... More
Requirement for an additional divalent metal cation to activate protein tyrosine kinases.
Authors:Sun G, Budde RJ
Journal:Biochemistry
PubMed ID:9047313
'In addition to the magnesium ion needed to form the true phosphate-donating substrate (ATP-Mg complex), we have determined that at least one additional Mg2+ ion is essential for the activation of protein tyrosine kinases. This activation was investigated in detail using purified Csk, Src, and the fibroblast growth factor receptor ... More
Ca2+ fluorescence imaging with pico- and femtosecond two-photon excitation: signal and photodamage.
Authors:Koester HJ, Baur D, Uhl R, Hell SW
Journal:Biophys J
PubMed ID:10512842
'The signal and limitations of calcium florescence imaging using nonresonant multiphoton absorption of near-infrared femto- and picosecond laser pulses were examined. The fluorescence changes of various Ca(2+)-indicators induced by transient increases of the intradendritic calcium concentration were evaluated by evoking physiological activity in neocortical neurons in rat brain slices. Photodamage ... More
Effects of magnesium on cyclic GMP hydrolysis by the bovine retinal rod cyclic GMP phosphodiesterase.
Authors:Srivastava D, Fox DA, Hurwitz RL
Journal:Biochem J
PubMed ID:7772055
'Knowledge of the kinetics of the rod cyclic GMP phosphodiesterase is essential for understanding the kinetics and gain of the light response. Therefore, the interactions between Mg2+, cyclic GMP, and purified, trypsin-activated bovine rod cyclic GMP phosphodiesterase (EC 3.1.4.17) were examined. The effects of Mg2+ and of cyclic GMP on ... More
Mechanisms of calcium influx into hippocampal spines: heterogeneity among spines, coincidence detection by NMDA receptors, and optical quantal analysis.
Authors:Yuste R, Majewska A, Cash SS, Denk W
Journal:J Neurosci
PubMed ID:10066251
'Dendritic spines receive most excitatory inputs in the vertebrate brain, but their function is still poorly understood. Using two-photon calcium imaging of CA1 pyramidal neurons in rat hippocampal slices, we investigated the mechanisms by which calcium enters into individual spines in the stratum radiatum. We find three different pathways for ... More