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Invitrogen
PREB Recombinant Rabbit Monoclonal Antibody (JE64-56)
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FIGURE: 1 / 3
PREB Antibody (MA5-50674) in IHC (P)
Immunohistochemistry of PREB in paraffin-embedded human lymph node tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with PREB recombinant monoclonal antibody (Product # MA5-50674) with a dilution of 1:400 for 1 hour at r... View More
Product Details
MA5-50674
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE64-56
Immunogen
Recombinant protein within human PREB aa 81-230/417.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive control: HepG2 cell lysates, human lymph nodes tissue, human colon tissue.
Predicted band size: 45 kDa
Subcellular Location: Endoplasmic reticulum membrane, Nucleus.
Target Information
Prolactin (PRL) expression in the pituitary is limited to specific cells. Pit-1 is a pituitary specific transcription factor that plays an important role in PRL expression, both in mature organism and during development. The PRL promoter contains numerous Pit-1 binding sites and these sites have been implicated in both basal level and kinase-mediated gene expression. The most proximal of these binding sites, termed 1P, has been shown to direct a response to numerous signals, such as cAMP and various G-proteins. A novel protein, termed PREB (prolactin regulatory element binding protein), has been recently identified that regulates PRL gene expression through the 1P site, though it contains no discernable DNA-binding motif. Recent studies suggest that PREB is encoded by a single-copy gene in both mice and humans and exhibits nuclear accumulation in pituitary cells. Evidence suggests that PREB is a novel transcription factor that assists in PRL expression whether alone, or in concert with Pit-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Guanine nucleotide-exchange factor SEC12; mammalian guanine nucleotide exchange factor mSec12; PREB; Prolactin regulatory element-binding protein; unnamed protein product; WD motif DNA-binding protein
Gene Aliases: PREB; SEC12
UniProt ID: (Human) Q9HCU5
Entrez Gene ID: (Human) 10113
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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