AmpliTaq™ DNA Polymerase with Buffer I or Buffer II

Citations & References (1798)

Applied Biosystems™

AmpliTaq™ DNA Polymerase with Buffer I or Buffer II

Name: AmpliTaq™ DNA Polymerase with Buffer I or Buffer II
SKU: N8080160

AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli.

Technical SupportCustomer Service

Change view

Catalog Number	Includes	Quantity
N8080160	Buffer I	250 Units
N8080161	Buffer II	250 Units
N8080172	Buffer II	1000 Units
N8080153	Buffer II	3,000 units
N8080156	Buffer II	5,000 units
N8080186	Buffer II	25,000 units
N8080152	Buffer I	3,000 units
N8080155	Buffer I	5,000 units
N8080171	Buffer I	1,000 units

9 Options

Catalog number N8080160

Price (JPY)

45,000

Each

Includes:

Buffer I

Quantity:

250 Units

Request bulk or custom format

AmpliTaq DNA Polymerase is a 94-kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process, and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial and lot-to-lot.

AmpliTaq DNA Polymerase is available with GeneAmp10X PCR Buffer I or with GeneAmp 10X PCR Buffer II and MgCl₂Solution.

Reliable and robust PCR

The thermal activity profile of AmpliTaq DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55–75°C). The enzyme's half-life is ∼40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications.

Features

The most thoroughly characterized enzyme available for the PCR process
Excellent thermal activity profile makes it good for PCR applications
QC-tested to guarantee reproducible results

Notes

For superior PCR performance, we recommend DreamTaq DNA Polymerase.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Exonuclease Activity5' - 3'

Fidelity (vs. Taq)1X

FormatStand-alone enzyme

Hot StartNo

IncludesBuffer I

No. of Reactions200 Reactions

Overhang3'-A

PolymeraseAmpliTaq DNA Polymerase

Product TypeDNA Polymerase

Quantity250 Units

Reaction FormatSeparate Components

Shipping ConditionDry Ice

Size (Final Product)5 kb or less

Starting MaterialDNA

Concentration5 U/μL

For Use With (Application)Standard PCR

Reaction SpeedStandard

Unit SizeEach

Contents & Storage

• AmpliTaq DNA Polymerase (5 U/μL), 50 μL
• GeneAmp 10X PCR Buffer, 1.5 mL

Store at -20°C.

Frequently asked questions (FAQs)

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

View all AmpliTaq™ DNA Polymerase with Buffer I or Buffer II FAQs

Citations & References (1798)

Citations & References

Abstract

Chimpanzee Fab fragments and a derived humanized immunoglobulin G1 antibody that efficiently cross-neutralize dengue type 1 and type 2 viruses.

Authors:Goncalvez AP; Men R; Wernly C; Purcell RH; Lai CJ

Journal:Journal of Virology

PubMed ID:

Passive immunization with monoclonal antibodies from humans or nonhuman primates represents an attractive alternative to vaccines for prevention of illness caused by dengue viruses (DENV) and other flaviviruses, including the West Nile virus. In a previous study, repertoire cloning to recover Fab fragments from bone marrow mRNA of chimpanzees infected ... More

Molecular genetics of tetrahydrobiopterin (BH4) deficiency in the Maltese population.

Authors:Farrugia R; Scerri CA; Montalto SA; Parascandolo R; Neville BG; Felice AE

Journal:Molecular Genetics and Metabolism

PubMed ID:

Deficient activity of the Dihydropteridine Reductase enzyme (DHPR; EC 1.5.1.34; OMIM 261630) is due to mutations in the Quinoid Dihydropteridine Reductase gene on 4p15.3 (QDPR; RefSeq NM_000320). It results in defective recycling of tetrahydrobiopterin (BH(4)) and homozygotes have a rare form of atypical Hyperphenylalaninaemia and Phenylketonuria (aPKU). The heterozygote frequency ... More

A specific structural requirement for ergosterol in long-chain fatty acid synthesis mutants important for maintaining raft domains in yeast.

Authors:Eisenkolb M; Zenzmaier C; Leitner E; Schneiter R

Journal:Molecular Biology of the Cell

PubMed ID:

Fungal sphingolipids contain ceramide with a very-long-chain fatty acid (C26). To investigate the physiological significance of the C26-substitution on this lipid, we performed a screen for mutants that are synthetically lethal with ELO3. Elo3p is a component of the ER-associated fatty acid elongase and is required for the final elongation ... More

Procollagen with skipping of alpha 1(I) exon 41 has lower binding affinity for alpha 1(I) C-telopeptide, impaired in vitro fibrillogenesis, and altered fibril morphology.

Authors:Cabral WA; Fertala A; Green LK; Korkko J; Forlino A; Marini JC

Journal:The Journal of Biological Chemistry

PubMed ID:

Previous in vitro data on type I collagen self-assembly into fibrils suggested that the amino acid 776-796 region of the alpha1(I) chain is crucial for fibril formation because it serves as the recognition site for the telopeptide of a docking collagen monomer. We used a natural collagen mutation with a ... More

Vibrio fischeri LuxS and AinS: comparative study of two signal synthases.

Authors:Lupp C; Ruby EG

Journal:Journal of Bacteriology

PubMed ID:

Vibrio fischeri possesses two acyl-homoserine lactone quorum-sensing systems, ain and lux, both of which are involved in the regulation of luminescence gene expression and are required for persistent colonization of the squid host, Euprymna scolopes. We have previously demonstrated that the ain system induces luminescence at cell densities that precede ... More

View all AmpliTaq™ DNA Polymerase with Buffer I or Buffer II citations