SNARF™-4F 5-(and-6)-Carboxylic Acid, Acetoxymethyl Ester, Acetate - Special Packaging

Citations & References (6)

Invitrogen™

SNARF™-4F 5-(and-6)-Carboxylic Acid, Acetoxymethyl Ester, Acetate - Special Packaging

The cell-permeant ratiometric pH indicator SNARF™-4F 5-(and-6)-carboxylic acid, acetoxymethyl ester, acetate (pKa of ∼6.4) exhibits a significant pH-dependent emission shiftRead more

Catalog Number	Quantity
S23921	20 x 50 μg

Catalog number S23921

Price (JPY)

116,900

Quantity:

20 x 50 μg

The cell-permeant ratiometric pH indicator SNARF™-4F 5-(and-6)-carboxylic acid, acetoxymethyl ester, acetate (pK_a of ∼6.4) exhibits a significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectively. This pH dependence allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection MethodFluorescence

Dye TypeFluorescent Dye-Based

IndicatorpH Indicator

Quantity20 x 50 μg

Shipping ConditionRoom Temperature

For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader

Product LineSNARF

Product TypepH Indicator

Unit SizeEach

Contents & Storage

Store in freezer -5°C to -30°C.

Frequently asked questions (FAQs)

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (6)

Citations & References

Abstract

Fluorescent indicators for intracellular pH.

Authors:Han J, Burgess K,

Journal:Chem Rev

PubMed ID:19831417

This review is about intracellular pH sensors, includingsmall fluorescent organic molecules, nanoparticles, andfluorescent proteins, e.g., GFP. It focuses on their preparations, photophysical properties, and advantages/disadvantagesfor intracellular pH measurements. The discussion is limitedto fluorescent indicators that have been applied to measureintracellular pH values since 1980. ... More

Synthesis and photophysical properties of new fluorinated benzo[c]xanthene dyes as intracellular pH indicators.

Authors:Liu J, Diwu Z, Leung WY

Journal:Bioorg Med Chem Lett

PubMed ID:11677123

Two new fluorinated benzo[c]xanthene dyes were synthesized by reaction of fluorinated 1,6-dihydroxynaphthalenes with 2,4- (and 2,5)-dicarboxy-3'-dimethylamino-2'-hydroxybenzophenone. The two critical fluorinated 1,6-dihydroxynaphthalene intermediates were prepared via a regioselective route. The fluorinated benzo[c]xanthene dyes exhibit desired lower pK(a) values (6.4 and 7.2, respectively) than their parent compound (pK(a)=7.5) while the pH-dependent dual-emission ... More

Tissue-specific regulation of sodium/proton exchanger isoform 3 activity in Na(+)/H(+) exchanger regulatory factor 1 (NHERF1) null mice. cAMP inhibition is differentially dependent on NHERF1 and exchange protein directly activated by cAMP in ileum versus proximal tubule.

Authors:Murtazina R, Kovbasnjuk O, Zachos NC, Li X, Chen Y, Hubbard A, Hogema BM, Steplock D, Seidler U, Hoque KM, Tse CM, De Jonge HR, Weinman EJ, Donowitz M,

Journal:J Biol Chem

PubMed ID:17580307

The multi-PDZ domain containing protein Na(+)/H(+) Exchanger Regulatory Factor 1 (NHERF1) binds to Na(+)/H(+) exchanger 3 (NHE3) and is associated with the brush border (BB) membrane of murine kidney and small intestine. Although studies in BB isolated from kidney cortex of wild type and NHERF1(-/-) mice have shown that NHERF1 ... More

Intracellular pH distribution in Saccharomyces cerevisiae cell populations, analyzed by flow cytometry.

Authors:Valli M, Sauer M, Branduardi P, Borth N, Porro D, Mattanovich D

Journal:Appl Environ Microbiol

PubMed ID:15746355

Intracellular pH has an important role in the maintenance of the normal functions of yeast cells. The ability of the cell to maintain this pH homeostasis also in response to environmental changes has gained more and more interest in both basic and applied research. In this study we describe a ... More

pH-Dependence of extrinsic and intrinsic H(+)-ion mobility in the rat ventricular myocyte, investigated using flash photolysis of a caged-H(+) compound.

Authors:Swietach P, Spitzer KW, Vaughan-Jones RD

Journal:Biophys J

PubMed ID:17056723

Passive H(+)-ion mobility within eukaryotic cells is low, due to H(+)-ion binding to cytoplasmic buffers. A localized intracellular acidosis can therefore persist for seconds or even minutes. Because H(+)-ions modulate so many biological processes, spatial intracellular pH (pH(i))-regulation becomes important for coordinating cellular activity. We have investigated spatial pH(i)-regulation in ... More

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