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인용 및 참조 문헌 (7)
Thermo Scientific™
EMCS (N-ε-malemidocaproyl-oxysuccinimide ester)
Thermo Scientific Pierce EMCS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| 22308 | 50 mg |
카탈로그 번호 22308
제품 가격(KRW)
560,000
Online offer
Ends: 31-Mar-2026
658,000할인액 98,000 (15%)
Each
수량:
50 mg
제품 가격(KRW)
560,000
Online offer
Ends: 31-Mar-2026
658,000할인액 98,000 (15%)
Each
Thermo Scientific Pierce EMCS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length spacer arm (9.4 angstroms).
Features of EMCS:
• Reactive groups: NHS ester and maleimide
• Reactive towards: amino and sulfhydryl groups
• C6-homolog of aliphatic spacer series which includes AMAS, BMPS, GMBS and EMCS
• NHS ester end couples with primary amines at pH 7-9 to form stable amide bonds
• Maleimides react with -SH groups at pH 6.5-7.5, forming stable thioether linkages
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-EMCS
• Increased sphere of coupling vs. GMBS
• Short aliphatic spacer has low potential for eliciting an immune response
Product References:
Crosslinker Application Guide -- search for recent literature references for this product
Features of EMCS:
• Reactive groups: NHS ester and maleimide
• Reactive towards: amino and sulfhydryl groups
• C6-homolog of aliphatic spacer series which includes AMAS, BMPS, GMBS and EMCS
• NHS ester end couples with primary amines at pH 7-9 to form stable amide bonds
• Maleimides react with -SH groups at pH 6.5-7.5, forming stable thioether linkages
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-EMCS
• Increased sphere of coupling vs. GMBS
• Short aliphatic spacer has low potential for eliciting an immune response
Product References:
Crosslinker Application Guide -- search for recent literature references for this product
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 투과성Yes
설명EMCS
형태Powder
라벨링 방법Chemical Labeling
분자량308.29
페길레이티드No
제품라인Pierce
수량50 mg
반응성 부분Maleimide, NHS Ester
배송 조건Ambient
용해도DMF, DMSO
스페이서 암 길이9.4 Å
수용성No
화학물질 반응성Amine-Sulfhydryl
CleavableNo
교차결합제 유형Heterobifunctional
형식Standard
제품 유형Crosslinker
스페이서Short (<10 Å)
Unit SizeEach
구성 및 보관
Upon receipt store desiccated at 4°C.
자주 묻는 질문(FAQ)
Can you provide the shelf-life for EMCS (N-ε-malemidocaproyl-oxysuccinimide ester)?
인용 및 참조 문헌 (7)
인용 및 참조 문헌
Abstract
Mechanism of reversal of phospholamban inhibition of the cardiac Ca2+-ATPase by protein kinase A and by anti-phospholamban monoclonal antibody 2D12.
Journal:J Biol Chem
PubMed ID:17548345
'Our model of phospholamban (PLB) regulation of the cardiac Ca(2+)-ATPase in sarcoplasmic reticulum (SERCA2a) states that PLB binds to the Ca(2+)-free, E2 conformation of SERCA2a and blocks it from transitioning from E2 to E1, the Ca(2+)-bound state. PLB and Ca(2+) binding to SERCA2a are mutually exclusive, and PLB inhibition of
Miniature single-particle immunoassay for prostate-specific antigen in serum using recombinant Fab fragments.
Journal:Clin Chem
PubMed ID:11067810
Quantitative, miniaturized nucleic acid assays and immunoassays can be developed with single microparticles, microfluorometric detection, and intrinsically fluorescent lanthanide chelates in a multiple assay format to decrease reagent consumption, cost, and assay time. We used recombinant Fab fragments to capture and detect free and total prostate-specific antigen (PSA) from serum
Characterizing phospholamban to sarco(endo)plasmic reticulum Ca2+-ATPase 2a (SERCA2a) protein binding interactions in human cardiac sarcoplasmic reticulum vesicles using chemical cross-linking.
Journal:J Biol Chem
PubMed ID:22247554
Chemical cross-linking was used to study protein binding interactions between native phospholamban (PLB) and SERCA2a in sarcoplasmic reticulum (SR) vesicles prepared from normal and failed human hearts. Lys(27) of PLB was cross-linked to the Ca(2+) pump at the cytoplasmic extension of M4 (at or near Lys(328)) with the homobifunctional cross-linker,
PNA microarrays for hybridisation of unlabelled DNA samples.
Journal:Nucleic Acids Res
PubMed ID:14500847
Several strategies have been developed for the production of peptide nucleic acid (PNA) microarrays by parallel probe synthesis and selective coupling of full-length molecules. Such microarrays were used for direct detection of the hybridisation of unlabelled DNA by time-of-flight secondary ion mass spectrometry. PNAs were synthesised by an automated process
Role of leucine 31 of phospholamban in structural and functional interactions with the Ca2+ pump of cardiac sarcoplasmic reticulum.
Journal:J Biol Chem
PubMed ID:15644311
The ability of two loss-of-function mutants, L31A and L31C, of phospholamban (PLB) to bind to and inhibit the Ca(2+) pump of cardiac sarcoplasmic reticulum (SERCA2a) was investigated using a molecular cross-linking approach. Leu(31) of PLB, located at the cytoplasmic membrane boundary, is a critical amino acid shown previously to be