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Invitrogen
GRP94 Polyclonal Antibody
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FIGURE: 1 / 14
GRP94 Antibody (36-2600) in ICC/IF
Immunofluorescence analysis of GRP94 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labelled with GRP94 Polyclonal Antibody (Product # 36-2600) at 5µg/mL in 0.1% BSA and incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor® 555 conjugate (Product # 36-2600)... View More
Product Details
36-2600
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Xenopus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from the N-terminal region of the mouse and human gp96 protein
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
GRP94 is a 803 amino acid protein belonging to the heat shock protein 90 family. It acts as a molecular chaperone that functions in the processing and transport of secreted proteins. GRP94 and its N-terminal fragment stimulates CTL expansion and maturation of human monocyte-derived dendritic cells (MDDC). It plays a central role in innate as well as acquired immunity, maturation and chemotaxis of dendritic cells, Ab production, cross-priming, as a potential marker in breast cancer and is a peptide acceptor in endoplasmic reticulum and an accessory to peptide loading of MHC class I molecules. Expression of GRP94 suppressed A23187-induced apoptosis and stabilized calcium homeostasis. GRP94 is expressed in melanoma or liver metastases of colon carcinoma cells, human gastric carcinoma BGC-823 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 94 kDa glucose-regulated protein; 94 kDa glucose-regulated protein {ECO:0000250|UniProtKB:P08113}; Endoplasmic reticulum resident protein 99; Endoplasmin; endoplasmin {ECO:0000250|UniProtKB:P08113}; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; ERp99; gp96 homolog; GRP-94; Heat shock protein 90 kDa beta member 1; heat shock protein 90 kDa beta member 1 {ECO:0000250|UniProtKB:P08113}; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; Heat shock protein family C member 4; hsp90b1 {ECO:0000250|UniProtKB:P08113}; Polymorphic tumor rejection antigen 1; stress-inducible tumor rejection antigen gp96; transforming growth factor alpha regulated gene 2; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen gp96; unnamed protein product
Gene Aliases: ECGP; endoplasmin; ERp99; GP96; GRP94; HEL-S-125m; HEL35; HSP90B1; HSPC4; TA-3; Targ2; Tra-1; TRA1
UniProt ID: (Human) P14625, (Mouse) P08113, (Rat) Q66HD0
Entrez Gene ID: (Human) 7184, (Mouse) 22027, (Rat) 362862
nucleotide binding
RNA binding
protein phosphatase inhibitor activity
calcium ion binding
protein binding
ATP binding
hydrolase activity
ATPase activity
protein phosphatase binding
protein binding involved in protein folding
low-density lipoprotein particle receptor binding
unfolded protein binding
ATP-dependent protein folding chaperone
virion binding
Hsp90 family chaperone
response to hypoxia
protein folding
protein transport
positive regulation of Wnt signaling pathway
retrograde protein transport, ER to cytosol
actin rod assembly
positive regulation of toll-like receptor signaling pathway
protein folding in endoplasmic reticulum
response to endoplasmic reticulum stress
ERAD pathway
negative regulation of apoptotic process
sequestering of calcium ion
cellular response to manganese ion
cellular response to ATP
protein localization to plasma membrane
response to stress
ER-associated ubiquitin-dependent protein catabolic process
regulation of phosphoprotein phosphatase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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