Immunocytochemistry of fixed and permeabilized F9 cells stained with 5 µg/mL Rat IgG2a K Isotype Control Purified (left) or 5 µg/mL Anti-Human/Mouse Sox2 Purified (right) followed by 10 µg/mL Anti-Rat IgG2a eFluor® 570. Nuclei are stained with DAPI (colocalization appears pink).

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Product Details

41-4817-82

Applications

Tested Dilution

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

10 µg/mL

Product Specifications

Species Reactivity

Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Secondary Antibody

Clone

r2a-21B2

Conjugate

eFluor™ 570 eFluor™ 570 eFluor™ 570

Excitation/Emission Max

556/569 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2573615

Target

IgG2a

Antibody Form

Whole Antibody

Product Specific Information

Description: The monoclonal antibody r2a-21B2 recognizes Rat IgG2a antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG2a or any mouse isotype antibodies.

Applications Reported: This R2a-21B2 antibody has been reported for use in immunocytochemistry, and immunohistochemical staining of frozen and formalin-fixed paraffin embedded tissue sections.

Applications Tested: This R2a-21B2 antibody has been tested by immunocytochemistry on fixed and permeabilized cells that have been stained first with a rat IgG2a anti-mouse antibody. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.

Excitation: 555 nm; Emission: 570 nm

Target Information

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Mouse anti-Rat IgG2a Secondary Antibody, eFluor™ 570, eBioscience™

Rat IgG2a Secondary Antibody (41-4817-82) in ICC/IF