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Invitrogen
ApoC3 Recombinant Rabbit Monoclonal Antibody (6H21L11)
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FIGURE: 1 / 2
ApoC3 Antibody (701238) in ICC/IF
Immunofluorescent analysis of ApoC3 in HepG2 cells using an ApoC3 recombinant rabbit monoclonal antibody (Product # 701238) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing subcellular cytoplasmic localization of Apo C3.
Product Details
701238
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
6H21L11
Immunogen
Peptide corresponding to amino acids 37-48 of human Apolipoprotein C3
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with mouse, non-human primate and rabbit based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Apolipoprotein C-III, also known as apo-CIII, is a protein that in humans is encoded by the APOC3 gene. Apo-CIII is a component of very low density lipoprotein (VLDL). APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to delay catabolism of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Apo-CIII; Apolipoprotein; apolipoprotein C-3; Apolipoprotein C-III; apolipoprotein C-III precursor; Apolipoprotein C3; MGC150353; unnamed protein product
Gene Aliases: Apo-C3; apo-CIII; ApoC-3; ApoC-III; APOC3; APOCIII
UniProt ID: (Human) P02656
Entrez Gene ID: (Human) 345, (Rat) 24207
lipid metabolic process
triglyceride metabolic process
lipid transport
G-protein coupled receptor signaling pathway
negative regulation of triglyceride catabolic process
negative regulation of very-low-density lipoprotein particle remodeling
negative regulation of very-low-density lipoprotein particle clearance
negative regulation of high-density lipoprotein particle clearance
negative regulation of low-density lipoprotein particle clearance
lipid catabolic process
triglyceride catabolic process
regulation of Cdc42 protein signal transduction
cholesterol efflux
phospholipid efflux
high-density lipoprotein particle remodeling
very-low-density lipoprotein particle assembly
chylomicron remnant clearance
lipoprotein metabolic process
cholesterol homeostasis
reverse cholesterol transport
negative regulation of fatty acid biosynthetic process
negative regulation of lipid metabolic process
negative regulation of receptor-mediated endocytosis
negative regulation of lipid catabolic process
negative regulation of cholesterol import
triglyceride homeostasis
triglyceride mobilization
inflammatory response
response to nutrient
cholesterol metabolic process
response to drug
lipoprotein transport
response to peptide hormone
negative regulation of lipoprotein lipase activity
cellular response to glucose stimulus
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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