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FIGURE: 1 / 3
VPS34 Antibody (710194) in ICC/IF
Immunofluorescent analysis of VPS34 in HeLa cells using a VPS34 Recombinant Rabbit Superclonal™ Antibody (Product # 710194) followed by detection using an Alexa Fluor 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing perinuclear as well as cytoplasmic localization of VPS34.
Product Details
710194
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
8HCLC
Immunogen
Peptide corresponding to amino acids 502-515 of human VPS34
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
PIK3C3 (hVPS34) is the sole class III member of the PI3 Kinase family. PIK3C3 phosphorylates phosphatidylinositol (PI) to produce phosphatidylinositol 3-phosphate [PI(3)P], which recruits downstream effectors containing FYVE and Phox domains to membranes. PIK3C3 is involved in coupling nutrient signaling to mTOR and also plays a role in autophagy and intracellular trafficking. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: catalytic subunit type 3 Antibody; Class III PI 3-kinase; hVps34; hVps34 Antibody; OTTMUSP00000023341; OTTMUSP00000023350; phosphatidylinositol 3-kinase; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; phosphoinositide-3-kinase, class 3; PI3-kinase type 3; PI3K type 3; pi3kc3; PIK3C3 Antibody; PtdIns-3-kinase type 3; unnamed protein product; vacuolar protein sorting 34 homolog
Gene Aliases: 5330434F23Rik; hVps34; PIK3C3; VPS34
UniProt ID: (Human) Q8NEB9, (Mouse) Q6PF93
Entrez Gene ID: (Human) 5289, (Mouse) 225326
nucleotide binding
protein kinase activity
protein binding
ATP binding
kinase activity
1-phosphatidylinositol-3-kinase activity
transferase activity
phosphotransferase activity, alcohol group as acceptor
phosphatidylinositol kinase activity
transferase activity, transferring phosphorus-containing groups
phosphatidylinositol 3-kinase activity
kinase
autophagosome assembly
pexophagy
protein targeting to lysosome
lipid metabolic process
endocytosis
autophagy
endosome organization
cellular response to starvation
regulation of autophagy
macroautophagy
regulation of macroautophagy
protein processing
regulation of cytokinesis
protein localization to pre-autophagosomal structure
phosphatidylinositol-3-phosphate biosynthetic process
cellular response to glucose starvation
response to leucine
protein kinase B signaling
positive regulation by host of viral genome replication
early endosome to late endosome transport
positive regulation of natural killer cell mediated cytotoxicity
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
synaptic vesicle endocytosis
cell division
interferon-gamma-mediated signaling pathway
interleukin-6-mediated signaling pathway
autophagosome maturation
positive regulation of protein lipidation
cytokinesis
protein phosphorylation
cell cycle
endosomal transport
phosphorylation
pexophagy
regulation of protein secretion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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