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Invitrogen
Claudin 1 Polyclonal Antibody (MH25)
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FIGURE: 1 / 92
Claudin 1 Antibody (71-7800) in IHC (P)
Immunohistochemistry analysis of Claudin-1 showing staining in the membrane and weak cytoplasm staining of paraffin-embedded human liver tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for ... View More
Product Details
71-7800
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Published species
Avian,
Bovine,
Chicken,
Dog,
Fish,
Human,
Mouse,
Pig,
Rabbit,
Rat,
Tag,
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Clone
MH25
Immunogen
A synthetic peptide derived from the C-terminus of the human/mouse Claudin-1 protein.
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with the ~ 22 kDa Claudin-1 protein. Reactivity was confirmed by western blotting and immunofluorescence. Positive controls include MDCK cells (dog), Caco-2cells (human), mouse liver, rat liver, and rat kidney.
NOTE: This antibody strongly cross reacts with the Claudin-3 protein. It has been observed in western blotting inhibition studies using Caco-2 cell lysates, that this antibody is inhibited by addition of Claudin-3 peptide. For a Claudin-1 specific antibody that does not cross-react with Claudin-3, please order Cat. No. 519000 (Rb x Claudin-1) instead.
Target Information
Claudin-1 is a member of the transmembrane protein family claudins located in cell-cell tight junctions and it acts as a co-receptor for HCV entry into hepatic cells. Claudins are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Claudin-1 is expressed in most tissues such as bladder, fallopian tube, liver, pancreas, prostate, and skin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Claudin-1; CLDN1; Senescence-associated epithelial membrane protein; senescence-associated epithelial membrane protein 1; tight junction transmembrane protein; unnamed protein product
Gene Aliases: AI596271; CLD1; CLDN1; ILVASC; SEMP1; UNQ481/PRO944
UniProt ID: (Human) O95832, (Rat) P56745, (Mouse) O88551
Entrez Gene ID: (Human) 9076, (Dog) 608207, (Rat) 65129, (Mouse) 12737
cell adhesion
establishment of blood-nerve barrier
response to toxic substance
calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules
positive regulation of cell migration
response to lipopolysaccharide
cell junction maintenance
maintenance of blood-brain barrier
cell-cell junction organization
response to ethanol
viral entry into host cell
protein oligomerization
positive regulation of epithelial cell proliferation involved in wound healing
establishment of skin barrier
xenobiotic transport across blood-nerve barrier
response to interleukin-18
bicellular tight junction assembly
cellular response to lead ion
cellular response to interferon-gamma
cellular response to tumor necrosis factor
response to dexamethasone
cellular response to transforming growth factor beta stimulus
positive regulation of wound healing
establishment of endothelial intestinal barrier
liver regeneration
positive regulation of bicellular tight junction assembly
cellular response to butyrate
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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