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Bethyl Laboratories
SUPT5H Polyclonal Antibody
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FIGURE: 1 / 1
SUPT5H Antibody (A300-868A) in IP
Detection of human SUPT5H by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SUPT5H antibody BL3555 (Product # A300-868A) used for IP at 3 µg/mg lysate. For blotting immunoprecipitated SUPT5H, rabbit anti-SUPT5H antibody BL3558 (Product #A300-869A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A300-868A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 50 and 100 of Human Suppressor of Ty 5 Homolog.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse and Orangutan
Target Information
Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DRB sensitivity-inducing factor 160 kDa subunit; DRB sensitivity-inducing factor large subunit; DSIF large subunit; DSIF p160; hSPT5; suppressor of Ty 5 homolog; Tat-cotransactivator 1 protein; Tat-CT1 protein; Transcription elongation factor SPT5; unnamed protein product
Gene Aliases: SPT5; SPT5H; SUPT5H; Tat-CT1
UniProt ID: (Human) O00267
Entrez Gene ID: (Human) 6829
negative regulation of transcription from RNA polymerase II promoter
DNA-templated transcription, elongation
regulation of transcription from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
positive regulation of macroautophagy
regulation of DNA-templated transcription, elongation
negative regulation of DNA-templated transcription, elongation
positive regulation of DNA-templated transcription, elongation
positive regulation of transcription elongation from RNA polymerase II promoter
regulation of transcription elongation from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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