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Bethyl Laboratories
PolA1 Polyclonal Antibody
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FIGURE: 1 / 1
PolA1 Antibody (A302-851A) in WB
Detection of human PolA1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PolA1 antibody (Product # A302-851A) used for WB at 0.04 µg/mL (A) and 0.4 µg/mL (B) and used for IP at 3 µg/mg lysate. PolA1 was also immunoprecipitated by rabbit anti-PolA1 antibody A302-850A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds ... View More
Product Details
A302-851A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1175 and 1225 of Human Polymerase (DNA directed), Alpha 1, Catalytic Subunit.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair they function to excise damaged DNA fragments and correct recombinational mismatches. These exonucleases include the family of DNA polymerases. DNA pol alpha, beta, delta and epsilon are involved in DNA replication and repair. DNA pol delta and DNA pol epsilon are multisubunit enzymes, with DNA pol delta consisting of two subunits: p125, which interacts with the sliding DNA clamp protein PCNA; and p50. The nuclear-encoded DNA pol gamma is the only DNA polymerase required for the replication of the mitochondrial DNA. DNA pol zeta is ubiquitously expressed in various tissues and mediates the cellular mechanism of damage-induced mutagenesis. DNA pol theta is a DNA polymerase-helicase that binds ATP and is involved in the repair of interstrand crosslinks.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA pol alpha; DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180; polymerase (DNA directed), alpha 1, catalytic subunit; polymerase (DNA) alpha 1, catalytic subunit; polymerase (DNA-directed), alpha (70kD); unnamed protein product
Gene Aliases: NSX; p180; PDR; POLA; POLA1; VEODS
UniProt ID: (Human) P09884
Entrez Gene ID: (Human) 5422
nucleotide binding
nucleic acid binding
DNA binding
chromatin binding
DNA replication origin binding
single-stranded DNA binding
DNA-directed DNA polymerase activity
protein binding
zinc ion binding
transferase activity
nucleotidyltransferase activity
protein kinase binding
DNA polymerase activity
metal ion binding
DNA synthesis involved in DNA repair
DNA replication
DNA-dependent DNA replication
DNA replication, synthesis of RNA primer
DNA replication initiation
DNA strand elongation involved in DNA replication
leading strand elongation
lagging strand elongation
DNA repair
nucleotide-excision repair
double-strand break repair via nonhomologous end joining
regulation of type I interferon production
mitotic DNA replication initiation
DNA synthesis involved in UV-damage excision repair
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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