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Invitrogen

IL-12 p70 Monoclonal Antibody (20C2)

Advanced Verification
2 Published Figures
21 References
View all (23) IL-12 p70 antibodies

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Product Details

M122

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-dependent
-

ELISA (ELISA)

Assay-dependent
View 19 publications 19 publications

Neutralization (Neu)

-
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human

Published species

Bacteria, Human

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

20C2

Immunogen

Recombinant human IL-12 (p70)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Amount

500 µg

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

RRID

AB_223556

Product Specific Information

M122 targets IL-12 in ELISA, and WB applications and shows reactivity with Human samples.



The M122 immunogen is recombinant human IL-12 (p70).



M122 detects IL-12 which has a predicted molecular weight of approximately 23 kDa.



The M122 IL12 antibody (clone 20C2) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M121B (biotinylated conjugate of clone C8.6) Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.



Antibody M122 (clone 20C2) and biotinylated antibody M121B (clone C8.6) have successfully been used in combination with recombinant IL12 p70 protein SIL12 in ELISA applications.



This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system's response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 20C2; CLMF p35; CLMF p40; cytotoxic lymphocyte maturation factor 1, p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12p70; IL-12 p75; IL-12 subunit p35; IL-12 subunit p40; IL-12, subunit p35; IL-12A; IL-12B; IL12; IL12, subunit p40; IL12A/B; IL12AB; IL35 subunit; ILN; Interleukin; interleukin 12, p35; interleukin 12, p40; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 alpha chain; interleukin-12 beta chain; Interleukin-12 p70; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; MGC151228; MGC151232; natural killer cell stimulatory factor 2; cytotoxic lymphocyte maturation factor 2; IL12p40; natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF/CLMF; IL12p35; 35kD; NKSF/CLMF; IL12p40; 40kD; NKSF1; NKSF2

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Gene Aliases: CLMF; CLMF2; IL-12A; IL-12B; IL12A; IL12B; IMD28; IMD29; NFSK; NKSF; NKSF1; NKSF2; P35

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UniProt ID: (Human) P29459, (Human) P29460

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Entrez Gene ID: (Human) 3592, (Human) 3593

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Function(s)
cytokine activity interleukin-12 receptor binding protein binding growth factor activity interleukin-12 beta subunit binding interleukin-27 binding protein heterodimerization activity cytokine receptor activity cytokine receptor binding interleukin-12 alpha subunit binding identical protein binding macromolecular complex binding interleukin-23 receptor binding
Process(es)
regulation of cytokine production positive regulation of leukocyte mediated cytotoxicity positive regulation of T cell mediated cytotoxicity positive regulation of defense response to virus by host natural killer cell activation involved in immune response positive regulation of lymphocyte mediated immunity positive regulation of T-helper 1 type immune response positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target negative regulation of inflammatory response to antigenic stimulus cell surface receptor signaling pathway JAK-STAT cascade cell proliferation response to UV-B cell migration natural killer cell activation negative regulation of interleukin-10 production negative regulation of interleukin-17 production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-17 production positive regulation of tumor necrosis factor production positive regulation of natural killer cell activation positive regulation of natural killer cell proliferation positive regulation of mononuclear cell proliferation positive regulation of tissue remodeling positive regulation of smooth muscle cell apoptotic process interleukin-12-mediated signaling pathway T-helper 1 type immune response T-helper cell differentiation T cell proliferation positive regulation of T cell proliferation positive regulation of activated T cell proliferation T cell activation defense response to protozoan positive regulation of memory T cell differentiation T-helper 1 cell differentiation positive regulation of osteoclast differentiation positive regulation of cell adhesion oocyte development negative regulation of smooth muscle cell proliferation positive regulation of lymphocyte proliferation negative regulation of protein secretion regulation of inflammatory response positive regulation of inflammatory response defense response to Gram-negative bacterium positive regulation of NK T cell activation positive regulation of NK T cell proliferation defense response to virus cellular response to lipopolysaccharide cellular response to interferon-gamma negative regulation of vascular endothelial growth factor signaling pathway positive regulation of NIK/NF-kappaB signaling negative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis positive regulation of T-helper 17 type immune response positive regulation of T-helper 17 cell lineage commitment immune response response to virus response to lipopolysaccharide positive regulation of tyrosine phosphorylation of STAT protein positive regulation of natural killer cell mediated cytotoxicity defense response to Gram-positive bacterium extrinsic apoptotic signaling pathway cellular response to virus positive regulation of dendritic cell chemotaxis
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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