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          • Primary Antibodies ›
          • NF1 Antibodies

          Invitrogen

          NF1 Monoclonal Antibody (McNFn27a)

          View all (16) NF1 antibodies

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          Cite NF1 Monoclonal Antibody (McNFn27a)

          Additional Information:
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          • Antibody Testing Data (2)
          NF1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          NF1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          NF1 Antibody (MA1-084) in ICC/IF

          Immunofluorescence analysis of Neurofibromin was performed using 70% confluent log phase Neuro-2A cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NF1 (McNFn27a) Mouse Monoclonal Antibody (Product # MA1-084) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (P... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          NF1 Antibody in Immunocytochemistry (ICC/IF)
          NF1 Antibody in Western Blot (WB)
          NF1 Monoclonal Antibody (McNFn27a)

          Product Details

          MA1-084

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          5-10 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          2 µg/mL
          -

          ELISA (ELISA)

          Assay-dependent
          -

          Immunoprecipitation (IP)

          Assay-dependent
          -

          Affinity Purification (AP)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          McNFn27a

          Immunogen

          Synthetic peptide corresponding to the N-terminal residues 27-41 of human neurofibromin.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          200 µg

          Purification

          Protein G

          Storage buffer

          PBS with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_560294

          Product Specific Information

          MA1-084 detects neurofibromin in human, rat and mouse cells.

          MA1-084 has been successfully used in Western blot, procedures. By Western blot, this antibody detects a ~230-260 kDa protein.

          The MA1-084 immunogen is a synthetic peptide corresponding to the N-terminal residues 27-41 of human neurofibromin.

          Target Information

          The protein encoded by this gene is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: DKFZp686J1293; FLJ21220; neurofibromatosis 1; Neurofibromatosis type 1; Neurofibromatosis-related protein NF-1; Neurofibromin; unnamed protein product

          View more View less

          Gene Aliases: AW494271; E030030H24Rik; Nf-1; NF1; NFNS; VRNF; WSS

          View more View less

          UniProt ID: (Human) P21359, (Mouse) Q04690, (Rat) P97526

          View more View less

          Entrez Gene ID: (Human) 4763, (Mouse) 18015, (Rat) 24592

          View more View less

          Function(s)
          GTPase activator activity protein binding lipid binding phosphatidylethanolamine binding phosphatidylcholine binding microtubule binding syndecan binding GTPase-activating protein
          Process(es)
          MAPK cascade angiogenesis osteoblast differentiation metanephros development response to hypoxia liver development endothelial cell proliferation negative regulation of endothelial cell proliferation positive regulation of endothelial cell proliferation regulation of cell-matrix adhesion negative regulation of cell-matrix adhesion negative regulation of leukocyte migration protein import into nucleus apoptotic process cell communication Ras protein signal transduction neurotransmitter secretion neuroblast proliferation negative regulation of neuroblast proliferation brain development peripheral nervous system development heart development skeletal muscle tissue development cell proliferation negative regulation of cell proliferation visual learning extrinsic apoptotic signaling pathway via death domain receptors regulation of gene expression negative regulation of Schwann cell proliferation glial cell proliferation Schwann cell proliferation Schwann cell development cell migration negative regulation of angiogenesis Rac protein signal transduction spinal cord development amygdala development forebrain astrocyte development neural tube development cerebral cortex development myelination in peripheral nervous system actin cytoskeleton organization extracellular matrix organization collagen fibril organization osteoclast differentiation adrenal gland development negative regulation of cell migration regulation of synaptic transmission, GABAergic mast cell apoptotic process positive regulation of mast cell apoptotic process myeloid cell apoptotic process positive regulation of myeloid cell apoptotic process cellular response to heat negative regulation of Rac protein signal transduction Schwann cell migration wound healing regulation of cell proliferation negative regulation of protein import into nucleus positive regulation of apoptotic process regulation of MAPK cascade negative regulation of MAPK cascade pigmentation protein kinase B signaling positive regulation of neuron apoptotic process regulation of blood vessel endothelial cell migration positive regulation of GTPase activity regulation of bone resorption negative regulation of osteoclast differentiation regulation of glial cell differentiation regulation of angiogenesis negative regulation of Ras protein signal transduction negative regulation of neurotransmitter secretion fibroblast proliferation negative regulation of fibroblast proliferation regulation of long-term neuronal synaptic plasticity sympathetic nervous system development camera-type eye morphogenesis astrocyte differentiation oligodendrocyte differentiation negative regulation of astrocyte differentiation negative regulation of oligodendrocyte differentiation smooth muscle tissue development hair follicle maturation artery morphogenesis forebrain morphogenesis regulation of developmental process cognition neuron apoptotic process negative regulation of glial cell proliferation long-term synaptic potentiation gamma-aminobutyric acid secretion, neurotransmission glutamate secretion, neurotransmission regulation of ERK1 and ERK2 cascade mast cell proliferation negative regulation of mast cell proliferation leukocyte apoptotic process stem cell proliferation extrinsic apoptotic signaling pathway in absence of ligand myeloid leukocyte migration observational learning regulation of postsynapse organization negative regulation of Schwann cell migration regulation of long-term synaptic potentiation regulation of intracellular signal transduction positive regulation of vascular smooth muscle cell proliferation vascular associated smooth muscle cell migration negative regulation of vascular associated smooth muscle cell migration vascular associated smooth muscle cell proliferation positive regulation of leukocyte apoptotic process negative regulation of stem cell proliferation positive regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of protein kinase activity signal transduction phosphatidylinositol 3-kinase signaling negative regulation of transcription factor import into nucleus regulation of GTPase activity negative regulation of MAP kinase activity regulation of neuron differentiation positive regulation of adenylate cyclase activity positive regulation of extrinsic apoptotic signaling pathway via death domain receptors
          It has to be done as per old AB suggested Products section.

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