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Invitrogen

p19ARF Monoclonal Antibody (5-C3-1)

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p19ARF Monoclonal Antibody (5-C3-1)

Product Details

MA1-16665

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunohistochemistry (Frozen) (IHC (F))

5 µg/mL
-

Immunocytochemistry (ICC/IF)

1:500-1:1,000
-

Flow Cytometry (Flow)

1 µg/mL
-

Immunoprecipitation (IP)

1:10-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

5-C3-1

Immunogen

A synthetic peptide containing amino acids 54-75 of the murine p19ARF/CDKN2A.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_568665

Product Specific Information

This antibody does not react with human or Golden hamster.

Armenian hamster has not been tested.

Target Information

In humans, the multiple tumor suppressor 1 locus (MTS1) encodes two unrelated genes, p16INK4a and p19ARF. Although both act as cell proliferation inhibitors, their mechanisms of action are different. p19ARF works as part of a p53-dependent pathway to counter uncontrolled proliferation and oncogenic signals. Mice lacking the p19ARF gene rapidly develop a broad spectrum of tumors. This result indicates that p19ARF is an important tumor suppressor. In addition, p19ARF helps prevent transforming signals from various onocoproteins via the p53 regulatory loop. These studies and others display the importance of p19ARF in cell cycle control and tumor suppression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: a frameshift between exon 1 (0.18) and exon 2 changed the ORF of p16INK4 gene.; Alternative reading frame; ARF; CDK4 inhibitor p16-INK4; CDK4I; cdkn2a; cdkn2a {ECO:0000312|EMBL:AAL76336.1, ECO:0000312|RGD:2323}; cdkn2a {ECO:0000312|EMBL:AAL76338.1, ECO:0000312|RGD:2323}; CDKN2A/ARF Intron 2 lncRNA; cell cycle inhibitor; cell cycle negative regulator beta; cell cycle regulator; Cyclin dependent kinase inhibitor 2A (p16, inhibits CDK4); Cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2a p16Ink4a; cyclin-dependent kinase inhibitor 2a p19Arf; cyclin-dependent kinase inhibitor 2A, isoform 1; cyclin-dependent kinase inhibitor 2A, isoform 2; cyclin-dependent kinase inhibitor 2A, isoform 3; cyclin-dependent kinase inhibitor 2A, isoforms 1/2; cyclin-dependent kinase inhibitor protein; hypothetical 18.1 kDa protein from longest open reading frame; inhibitor of cdk4 A; mitochondrial smARF; MTS-1; Multiple tumor suppressor 1; multiple tumour suppressor 1; p14ARF; p16; p16 gamma; p16-INK4; p16-INK4a; p19ARF; Tumor suppressor ARF

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Gene Aliases: ARF; ARF-INK4a; CAI2; CDK4I; CDKN2; CDKN2A; CMM2; INK4; INK4A; INK4a-ARF; Ink4a/Arf; MLM; MTS-1; MTS1; P14; P14ARF; P16; p16(INK4a); P16-INK4A; p16Cdkn2a; P16INK4; P16INK4A; P19; p19; P19ARF; Pctr1; TP16

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UniProt ID: (Human) P42771, (Mouse) P51480, (Rat) Q9R0Z3

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Entrez Gene ID: (Human) 1029, (Mouse) 12578, (Rat) 25163

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Function(s)
p53 binding DNA binding RNA binding cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding SUMO transferase activity protein kinase binding NF-kappaB binding ligase inhibitor activity ubiquitin-protein transferase inhibitor activity RNA polymerase II sequence-specific DNA binding transcription factor binding MDM2/MDM4 family protein binding disordered domain specific binding ubiquitin ligase inhibitor activity transcription factor activity, sequence-specific DNA binding transcription factor binding poly(A) RNA binding protein N-terminus binding
Process(es)
regulation of cyclin-dependent protein serine/threonine kinase activity G1/S transition of mitotic cell cycle protein polyubiquitination mitophagy negative regulation of cell-matrix adhesion transcription, DNA-templated regulation of transcription, DNA-templated rRNA processing negative regulation of protein kinase activity apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process cell cycle cell cycle arrest aging cell aging negative regulation of cell proliferation epidermis development apoptotic mitochondrial changes rRNA transcription response to organonitrogen compound regulation of G2/M transition of mitotic cell cycle regulation of gene expression response to organic cyclic compound negative regulation of cell growth negative regulation of B cell proliferation regulation of protein stability protein destabilization negative regulation of NF-kappaB transcription factor activity negative regulation of protein binding negative regulation of immature T cell proliferation in thymus positive regulation of protein sumoylation negative regulation of mammary gland epithelial cell proliferation positive regulation of smooth muscle cell apoptotic process somatic stem cell population maintenance senescence-associated heterochromatin focus assembly negative regulation of phosphorylation response to drug glucose homeostasis positive regulation of apoptotic process positive regulation of DNA damage response, signal transduction by p53 class mediator negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of nucleocytoplasmic transport regulation of protein export from nucleus somatic stem cell division protein stabilization positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of ubiquitin-protein transferase activity mitochondrial depolarization positive regulation of apoptotic process involved in mammary gland involution cellular response to hydrogen peroxide protein K63-linked ubiquitination positive regulation of cell cycle arrest cellular senescence replicative senescence positive regulation of protein localization to nucleus positive regulation of signal transduction by p53 class mediator regulation of apoptotic DNA fragmentation negative regulation of proteolysis involved in cellular protein catabolic process regulation of protein targeting to mitochondrion negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of macrophage apoptotic process positive regulation of cellular senescence Ras protein signal transduction response to organic substance cerebellum development negative regulation of transcription from RNA polymerase II promoter positive regulation of gene expression protein sumoylation keratinocyte differentiation nuclear body organization mammary gland epithelial cell proliferation protein localization to nucleus keratinocyte proliferation regulation of cell cycle apoptotic process involved in mammary gland involution oncogene-induced cell senescence apoptotic signaling pathway protein localization to nucleolus negative regulation of ubiquitin protein ligase activity amyloid fibril formation regulation of G1/S transition of mitotic cell cycle negative regulation of protein neddylation
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