Immunohistochemical analysis of paraffin-embedded human kidney cancer tissues using IRAK4 monoclonal antibody (Product # MA5-15883) followed with DAB staining.

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IRAK4 Antibody — Figure 4. IRAK4 kinase inhibition stabilizes TLR-induced myddosome interactions. A , BMDMs were pretreated with either DMSO or 20 mu m I4 inhb for 30 min. Cells were then left untreated or stimulated with 1 mug/ml LPS for a further 30 min. WCLs were then subjected to MyD88 IP followed by immunoblotting for IRAK4, IRAK1, P-IRAK4, and MyD88 or directly subjected to immunoblotting with the same antibodies. MyD88 immunoblotting also acted as a loading control. The data presented are representative of three independent experiments. B , WT iBMDMs were treated as in A , before WCLs were subjected to MyD88 IP followed by immunoblotting for IRAK4, IRAK1, and MyD88 or directly subjected to immunoblotting with the same antibodies. The data presented are representative of three independent experiments. C , BMDMs were pretreated with either DMSO or 20 mu m I4 inhb for 30 min. Cells were then left untreated or stimulated with either 500 ng/ml P3C or 0.5 mu m CpG DNA 1826 for a further 30 min. WCLs were then subjected to MyD88 IP followed by immunoblotting for IRAK4, IRAK1, P-IRAK4, and MyD88 or directly subjected to immunoblotting with the same antibodies. MyD88 immunoblotting also acted as a loading control. The data presented are representative of three independent experiments. D , Irak4 -/- iBMDMs expressing IRAK4-mCitrine were treated as in A before WCLs were subjected to GFP IP, followed by immunoblotting for MyD88 and GFP, or directly subjected to immunoblotting for P-IRAK4, IRAK1, {TM}

Product Details

MA5-15883

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

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Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Flow Cytometry (Flow)

1:200-1:400

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2H9

Immunogen

Purified recombinant fragment of human IRAK4 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11154205

Product Specific Information

MA5-15883 targets IRAK4 in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with Human, mouse, and Non-human primate samples.

The MA5-15883 immunogen is purified recombinant fragment of human IRAK4 expressed in E. Coli. .

MA5-15883 detects IRAK4 which has a predicted molecular weight of approximately 52kDa.

Target Information

IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. Overexpression of IRAK-4 activates NF-kappa-B and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: interleukin-1; Interleukin-1 receptor-associated kinase 4; IRAK-4; Renal carcinoma antigen NY-REN-64

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Gene Aliases: 8430405M07Rik; 9330209D03Rik; IPD1; IRAK-4; IRAK4; NY-REN-64; REN64

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UniProt ID: (Human) Q9NWZ3, (Mouse) Q8R4K2

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Entrez Gene ID: (Human) 51135, (Mouse) 266632

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Function(s)

magnesium ion binding protein kinase activity protein serine/threonine kinase activity interleukin-1 receptor binding protein binding ATP binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

cytokine production toll-like receptor signaling pathway neutrophil mediated immunity MyD88-dependent toll-like receptor signaling pathway JNK cascade cytokine-mediated signaling pathway toll-like receptor 9 signaling pathway positive regulation of I-kappaB kinase/NF-kappaB signaling innate immune response positive regulation of smooth muscle cell proliferation neutrophil migration immune system process protein phosphorylation signal transduction phosphorylation intracellular signal transduction

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IRAK4 Monoclonal Antibody (2H9)

IRAK4 Antibody (MA5-15883) in IHC (P)

Product Details

MA5-15883

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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