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Invitrogen
PGK1 Recombinant Rabbit Monoclonal Antibody (4B3Q3)
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FIGURE: 1 / 1
PGK1 Antibody (MA5-35173) in WB
Western blot analysis of PGK1 in extracts of various cell lines. Samples were incubated with PGK1 Monoclonal antibody (Product # MA5-35173) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
MA5-35173
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4B3Q3
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PGK1 (P00558)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MSLSNKLTLD KLDVKGKRVV MRVDFNVPMK NNQITNNQRI KAAVPSIKFC LDNGAKSVVL MSHLGRPDGV PMPDKYSLEP VAVELKSLLG KDVLFLKDCV
Target Information
Also known as ATP:3-phosphoglycerate 1-phosphotransferase showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell migration-inducing gene 10 protein; epididymis secretory sperm binding protein Li 68p; MIG10; PGK1; Phosphoglycerate kinase 1; Primer recognition protein 2; PRP 2; unnamed protein product
Gene Aliases: HEL-S-68p; MIG10; OK/SW-cl.110; Pgk; Pgk-1; PGK1; PGKA
UniProt ID: (Human) P00558, (Mouse) P09411, (Rat) P16617
Entrez Gene ID: (Human) 5230, (Mouse) 18655, (Rat) 24644
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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