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Invitrogen
TFAM Recombinant Rabbit Monoclonal Antibody (4H2M9)
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FIGURE: 1 / 4
TFAM Antibody (MA5-35365) in IHC (P)
Immunohistochemistry analysis of TFAM in paraffin-embedded human esophageal cancer. Samples were incubated with TFAM Monoclonal antibody (Product # MA5-35365) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Details
MA5-35365
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4H2M9
Immunogen
Synthetic peptide
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: QDAYRAEWQV YKEEISRFKE QLTPSQIMSL EKEIMDKHLK RKAMTKKKEL TLLGKPKRPR SAYNVYVAER FQEAKGDSPQ EKLKTVKENW KNLSDSEKEL Y
Target Information
Mitochondrial transcription factor A (TFAM) is a crucial protein encoded by nuclear genes, but it is transported from the cytoplasm to mitochondria where it plays a key role in the maintenance and expression of mitochondrial DNA (mtDNA). It regulates the replication and transcription of mtDNA, ensuring the proper functioning of mitochondrial processes. Structurally, TFAM contains a molecular region known as the LC3 interacting region (LIR) motif, which allows it to bind to autophagy proteins such as LC3. This interaction is essential for the autolysosomal pathway, helping to eliminate leaked mtDNA and limit inflammation. TFAM levels vary across tissues, with notable effects on mitochondrial function; high levels can repress mtDNA expression in certain tissues, such as skeletal muscle, and contribute to deficiencies in oxidative phosphorylation (OXPHOS). Conversely, in other tissues like the heart, increased mtDNA copy number maintains a balanced TFAM-to-mtDNA ratio, preserving OXPHOS capacity. TFAM is integral to the packaging, stability, and replication of the mitochondrial genome, and disruptions in TFAM can have severe effects, including implications in neurodegeneration and other diseases related to mtDNA stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Mitochondrial transcription factor 1; mitochondrial transcription factor A; MtTF1; mtTFA; TCF-6; Testis-specific high mobility group protein; testis-specific HMG-box protein m-tsHMG; TFAM; Transcription factor 6; transcription factor 6-like 1; Transcription factor 6-like 2; transcription factor 6-like 2 (mitochondrial transcription factor); transcription factor 6-like 3; Transcription factor A, mitochondrial; TS-HMG; unnamed protein product
Gene Aliases: AI661103; Hmgts; MTDPS15; MTTF1; MTTFA; TCF6; TCF6L1; TCF6L2; TCF6L3; TFAM; tsHMG
UniProt ID: (Human) Q00059, (Rat) Q91ZW1, (Mouse) P40630
Entrez Gene ID: (Human) 7019, (Rat) 83474, (Mouse) 21780
mitochondrial RNA polymerase regulatory region DNA binding
transcription coactivator binding
DNA binding
chromatin binding
RNA binding
protein binding
heat shock protein binding
mitochondrial RNA polymerase binding promoter specificity activity
sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription factor activity, sequence-specific DNA binding
DNA binding, bending
poly(A) RNA binding
mitochondrial light strand promoter sense binding
mitochondrial DNA replication
chromatin remodeling
regulation of transcription, DNA-templated
transcription from mitochondrial promoter
transcription initiation from mitochondrial promoter
mitochondrial respiratory chain complex assembly
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
response to hypoxia
response to nutrient
transcription, DNA-templated
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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