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Invitrogen
ATP2B1/2/3 Polyclonal Antibody
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FIGURE: 1 / 5
ATP2B1/2/3 Antibody (PA5-104020) in ICC/IF
Immunocytochemistry analysis of ATP2B1/2/3 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ATP2B1/2/3 polyclonal antibody (Product # PA5-104020) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat... View More
Product Details
PA5-104020
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ATP2B3(Accession Q16720), corresponding to amino acid residues I1005-I1055.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total ATP2B1/2/3.
Target Information
The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 3. Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATPase, Ca++ transporting, plasma membrane 3; cilia and flagella associated protein 39; plasma membrane calcium ATPase; Plasma membrane calcium ATPase isoform 3; plasma membrane calcium pump; Plasma membrane calcium pump isoform 3; plasma membrane calcium-ATPase isoform 3, splice variant a at alternative splice site C; Plasma membrane calcium-transporting ATPase 3; PMCA3; unnamed protein product
Gene Aliases: 6430519O13Rik; ATP2B3; CFAP39; CLA2; OPCA; PMCA3; PMCA3a; SCAX1
UniProt ID: (Human) Q16720, (Rat) Q64568
Entrez Gene ID: (Human) 492, (Rat) 29599, (Mouse) 320707
nucleotide binding
calcium-transporting ATPase activity
protein binding
calmodulin binding
ATP binding
calcium ion transmembrane transporter activity
ATPase activity
metal ion binding
P-type calcium transporter activity involved in regulation of presynaptic cytosolic calcium ion concentration
PDZ domain binding
primary active transporter
ion transport
calcium ion transport
ion transmembrane transport
regulation of cytosolic calcium ion concentration
calcium ion transmembrane transport
regulation of presynaptic cytosolic calcium ion concentration
regulation of cardiac conduction
calcium ion export from cell
neural retina development
brain development
metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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