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Invitrogen

Phospho-ULK1 (Ser317) Polyclonal Antibody

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Phospho-ULK1 (Ser317) Polyclonal Antibody

Product Details

PA5-104556

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human ULK1(Accession O75385), corresponding to amino acid residues around phosphorylated Ser317.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2816031

Product Specific Information

Antibody detects endogenous levels of ULK1 only when phosphorylated at Ser317.

Target Information

ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: APG1; ATG 1; ATG1; ATG1 autophagy related 1 homolog; Autophagy-related protein 1 homolog; C. elegans; EC:2.7.11.1; FLJ38455; Serine/threonine-protein kinase ULK1; Serine/threonine-protein kinase Unc51.1; ULK 1; UNC 51; Unc-51-like kinase 1

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Gene Aliases: ATG1; ATG1A; AU041434; hATG1; KIAA0722; mKIAA0722; ULK1; UNC51; Unc51.1

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UniProt ID: (Human) O75385, (Mouse) O70405

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Entrez Gene ID: (Human) 8408, (Rat) 360827, (Mouse) 22241

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Function(s)
nucleotide binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding kinase activity transferase activity small GTPase binding identical protein binding macromolecular complex binding GTPase binding protein serine kinase activity molecular_function Rab GTPase binding protein kinase binding protein complex binding transferase activity, transferring phosphorus-containing groups Hsp90 protein binding non-receptor serine/threonine protein kinase
Process(es)
autophagosome assembly mitophagy protein phosphorylation ubiquitin-dependent protein catabolic process autophagy signal transduction axonogenesis protein localization negative regulation of cell proliferation regulation of autophagy positive regulation of autophagy regulation of tumor necrosis factor-mediated signaling pathway macroautophagy regulation of macroautophagy peptidyl-serine phosphorylation neuron projection regeneration neuron projection development negative regulation of protein complex assembly cellular response to nutrient levels cellular response to stress piecemeal microautophagy of nucleus response to starvation protein autophosphorylation negative regulation of collateral sprouting axon extension reticulophagy protein-containing complex assembly positive regulation of autophagosome assembly Ras protein signal transduction positive regulation of macroautophagy cerebellar granule cell differentiation radial glia guided migration of cerebellar granule cell receptor internalization regulation of neurotrophin TRK receptor signaling pathway regulation of gene expression regulation of cell death phosphorylation mitophagy in response to mitochondrial depolarization
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