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Invitrogen

Phospho-PKA alpha/beta/gamma (Thr198) Polyclonal Antibody

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View all (5) PKA alpha/beta/gamma antibodies

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Product Details

PA5-105515

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human PRKACA(Accession P17612), corresponding to amino acid residues around phosphorylated Thr198.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2816943

Product Specific Information

Antibody detects endogenous levels of PKA alpha/beta/gamma CAT only when phosphorylated at Thr198.

Target Information

Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc; Protein Kinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit - PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C-alpha-s; alternatively spliced isoform; cAMP-dependent protein kinase C beta; cAMP-dependent protein kinase catalytic subunit alpha; cAMP-dependent protein kinase catalytic subunit beta; cAMP-dependent protein kinase catalytic subunit C alpha; cAMP-dependent protein kinase catalytic subunit gamma; PKA C-alpha; PKA C-beta; PKA C-gamma; protein kinase A; protein kinase A catalytic subunit; protein kinase A catalytic subunit beta; protein kinase, cAMP-dependent, alpha catalytic subunit; protein kinase, cAMP-dependent, beta catalytic subunit; protein kinase, cAMP-dependent, catalytic, alpha; protein kinase, cAMP-dependent, catalytic, beta; protein kinase, cAMP-dependent, catalytic, gamma; protein kinase, cAMP-dependent, gamma catalytic subunit; serine(threonine) protein kinase; sperm cAMP-dependent protein kinase catalytic subunit; unnamed protein product

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Gene Aliases: BDPLT19; CAFD1; CAFD2; Cs-PKA; KAPG; PKA C-beta; PKACA; PKACB; PKACg; PKCA1; PKCD; PPNAD4; PRKACA; PRKACB; PRKACG

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UniProt ID: (Human) P17612, (Human) P22694, (Human) P22612, (Mouse) P05132, (Rat) P68182, (Mouse) P68181

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Entrez Gene ID: (Human) 5566, (Human) 5567, (Human) 5568, (Rat) 25636, (Mouse) 18747, (Rat) 293508, (Mouse) 18749

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Function(s)
nucleotide binding magnesium ion binding protein kinase activity protein serine/threonine kinase activity cAMP-dependent protein kinase activity protein serine/threonine/tyrosine kinase activity protein binding ATP binding kinase activity transferase activity protein kinase binding protein domain specific binding manganese ion binding ubiquitin protein ligase binding protein kinase A regulatory subunit binding channel activator activity protein serine kinase activity Rab GTPase binding protein complex binding transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase
Process(es)
mesoderm formation neural tube closure regulation of heart rate renal water homeostasis mRNA processing protein phosphorylation protein export from nucleus adenylate cyclase-activating G-protein coupled receptor signaling pathway adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion regulation of macroautophagy peptidyl-serine phosphorylation cytokine-mediated signaling pathway cellular potassium ion homeostasis cellular response to nutrient levels positive regulation of insulin secretion negative regulation of interleukin-2 production high-density lipoprotein particle assembly cellular response to heat mitochondrial protein catabolic process interleukin-2-mediated signaling pathway TORC1 signaling proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of cholesterol biosynthetic process regulation of osteoblast differentiation positive regulation of gluconeogenesis negative regulation of smoothened signaling pathway positive regulation of protein export from nucleus sperm capacitation positive regulation of phagocytosis modulation of synaptic transmission positive regulation of calcium-mediated signaling regulation of cell cycle regulation of cardiac muscle contraction regulation of proteasomal protein catabolic process cellular response to cold regulation of protein processing cellular response to glucose stimulus cellular response to parathyroid hormone stimulus cellular response to glucagon stimulus cellular response to epinephrine stimulus cell communication by electrical coupling involved in cardiac conduction vascular endothelial cell response to laminar fluid shear stress postsynaptic modulation of chemical synaptic transmission cAMP/PKA signal transduction regulation of cardiac conduction negative regulation of TORC1 signaling negative regulation of glycolytic process through fructose-6-phosphate protein localization to lipid particle regulation of bicellular tight junction assembly signal transduction adenylate cyclase-modulating G-protein coupled receptor signaling pathway spermatogenesis male gonad development positive regulation of cell proliferation peptidyl-threonine phosphorylation regulation of cellular respiration protein autophosphorylation negative regulation of meiotic cell cycle regulation of synaptic transmission, glutamatergic positive regulation of cell cycle arrest negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning phosphorylation response to clozapine
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